Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-03-13 DOI:10.1016/j.xpro.2025.103692
Olga Vafaeva, Poommaree Namchaiw, Karl Murray, Elva Diaz, Hwai-Jong Cheng
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引用次数: 0

Abstract

The neurosphere assay is the gold standard for assessing the proliferative and differentiation capacities of neural progenitor cells (NPCs). Here, we present a protocol for isolating, propagating, and maintaining hippocampal neurospheres from adult and aged mice and differentiating cultured NPCs into neurons and astrocytes. We describe steps for establishing a heterochronic co-culture of neurosphere-derived cells with primary neurons. Using neurospheres from old animals enables investigation of the effects of aging on the development and differentiation of newborn neurons.

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神经球试验是评估神经祖细胞(NPC)增殖和分化能力的黄金标准。在这里,我们介绍了一种从成年和老年小鼠体内分离、繁殖和维持海马神经球,并将培养的神经祖细胞分化成神经元和星形胶质细胞的方法。我们描述了建立神经球衍生细胞与原代神经元异时共培养的步骤。使用老年动物的神经球可以研究衰老对新生神经元发育和分化的影响。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
期刊介绍:
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Protocol for high-precision CRISPR-Cas12a-based SNV detection on synthetic DNA, cell line cfDNA models, and liquid biopsies. Protocol for mouse carotid artery perfusion for in situ brain tissue fixation and parallel unfixed tissue collection. Protocol for preparing mouse hippocampal slices for ex vivo recordings of the temporoammonic pathway. Protocol for chromatin immunoprecipitation of chromatin-binding proteins in Schizosaccharomyces pombe using a dual-crosslinking approach. Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus.
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