Effect of viscosity in the medium for sperm cryopreservation of golden eagle (Aquila chrysaetos).

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-11-01

J A Herrera-Barragan, S S Landa-Garcia, F G Sill, J J Perez-Rivero

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引用次数: 0

Abstract

Background: The development of assisted reproduction techniques for birds is useful for ex situ conservation but is limited. For the golden eagle (Aquila chrysaetos), only artificial insemination procedures using extenders developed over 50 years ago have been described.

Objective: To evaluate the effect of the viscosity of the cryopreservation medium on the acrosomal reaction ability of A. chrysaetos sperm.

Materials and methods: Viscosity was determined in 45 ejaculates. A design using the Lake medium, 6 % dimethylacetamide (DMA), and Ficoll (10 %, 35 %, and 45 %) was developed to create conditions of viscosity that was lower, similar, and higher than those determined in fresh semen.

Results: The viscosity of fresh semen was 3.2320 mPa/s. In aliquots of the Lake medium supplemented with 6 % DMA and 10 %, 35 %, and 45 % Ficoll, the viscosities were 2.1698 mPa/s, 3.5393 mPa/s, and 6.1123 mPa/s, respectively. Post-thaw, in the aliquot with 10 % Ficoll, 74 % of sperm were alive, with 28 % mobility, and 21 % exhibited an acrosomal reaction with percentages that were significantly lower (P < 0.05) than those observed in sperm frozen with 30 % and 45 % Ficoll.

Conclusion: Viscosity has a positive influence on the viability of cryopreserved semen. https://doi.org/10.54680/fr24610110512.

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背景：鸟类辅助繁殖技术的发展对异地保护很有帮助，但却很有限。对于金雕（Aquila chrysaetos）来说，只有50多年前开发的使用延长剂的人工授精程序被描述过：评估冷冻保存介质的粘度对金雕精子顶体反应能力的影响：材料和方法：测定 45 个射精的粘度。使用 Lake 培养基、6% 二甲基乙酰胺（DMA）和 Ficoll（10%、35% 和 45%）进行设计，以创造比新鲜精液中测定的粘度更低、相似和更高的条件：结果：新鲜精液的粘度为 3.2320 mPa/s。在添加了 6 % DMA 和 10 %、35 % 和 45 % Ficoll 的等分湖培养基中，粘度分别为 2.1698 mPa/s、3.5393 mPa/s 和 6.1123 mPa/s。解冻后，在含 10% Ficoll 的等分试样中，74% 的精子是活的，28% 的精子具有活动能力，21% 的精子出现顶体反应，其百分比明显低于含 30% 和 45% Ficoll 的冷冻精子（P < 0.05）：https://doi.org/10.54680/fr24610110512。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.