Anti-inflammatory, antioxidant, and acute toxicity of Brugmansia arborea extracts from wild plants and shoots obtained by indirect organogenesis. A thermographic assay to anti-inflammatory evaluation
Mariana Zuleima Pérez-González , María del Carmen Juárez-Vázquez , Mariana Sánchez-Ramos , Leonardo Moreno-Villalba , María Adelina Jiménez-Arellanes
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引用次数: 0
Abstract
Inflammation is a condition that affects a large percentage of the population, rendering the search for alternative treatments urgent. Medicinal plant such as Brugmansia arborea has been employed in traditional medicine, it is used to alleviate menstrual pain, acne, promote wound healing, and ease joint pain, headaches, and other ailments. Some alkaloids (mainly atropine, scopolamine, and nor-hyoscyamine) have been described. In this paper, the in-vitro cultivation from leaf explants of B. arborea through indirect organogenesis is described. From wild plants (BA) and shoots (BAB) their extracts were prepared, and were analyzed by thin-layer chromatography and gas chromatography-mass spectrometry, identifying 18 main metabolites. Antioxidant activity was assessed with EC50 = 199.52 and 31.63 mg/mL for BA and BAB, respectively. Boths extracts showed a DL50 > 2 g/kg and is classified as non-toxic. In Acetylated and Hydrolyzed extracts, acetyl-rutin and kaempferol, respectively were identified. Anti-inflammatory activity was assayed employing carrageenan model. The ED50 values were < 158.48 mg/kg by BA, BAB, Ac-BA, and H-BA, being more active the hydrolyzed extract (ED50 = 63.09 and 64.56 mg/kg) for females and male mice. In topical TPA model, the ED50 value were < 0.47 mg/ear for BA, BAB, Ac-BA, and H-BA. Thermographic analysis is a non-invasive, cheap, effective and reproducible method to evaluate the anti-inflammatory effect of substances, since it quantifies the increase in temperature in an inflammatory process, so this technique constitutes an alternative in the search for this type of agent.
期刊介绍:
Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.