Anti-inflammatory, antioxidant, and acute toxicity of Brugmansia arborea extracts from wild plants and shoots obtained by indirect organogenesis. A thermographic assay to anti-inflammatory evaluation

IF 6.9 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Biomedicine & Pharmacotherapy Pub Date : 2025-03-17 DOI:10.1016/j.biopha.2025.117972
Mariana Zuleima Pérez-González , María del Carmen Juárez-Vázquez , Mariana Sánchez-Ramos , Leonardo Moreno-Villalba , María Adelina Jiménez-Arellanes
{"title":"Anti-inflammatory, antioxidant, and acute toxicity of Brugmansia arborea extracts from wild plants and shoots obtained by indirect organogenesis. A thermographic assay to anti-inflammatory evaluation","authors":"Mariana Zuleima Pérez-González ,&nbsp;María del Carmen Juárez-Vázquez ,&nbsp;Mariana Sánchez-Ramos ,&nbsp;Leonardo Moreno-Villalba ,&nbsp;María Adelina Jiménez-Arellanes","doi":"10.1016/j.biopha.2025.117972","DOIUrl":null,"url":null,"abstract":"<div><div>Inflammation is a condition that affects a large percentage of the population, rendering the search for alternative treatments urgent. Medicinal plant such as <em>Brugmansia arborea</em> has been employed in traditional medicine, it is used to alleviate menstrual pain, acne, promote wound healing, and ease joint pain, headaches, and other ailments. Some alkaloids (mainly atropine, scopolamine, and nor-hyoscyamine) have been described. In this paper, the <em>in-vitro</em> cultivation from leaf explants of <em>B. arborea</em> through indirect organogenesis is described. From wild plants (BA) and shoots (BAB) their extracts were prepared, and were analyzed by thin-layer chromatography and gas chromatography-mass spectrometry, identifying 18 main metabolites. Antioxidant activity was assessed with EC<sub>50</sub> = 199.52 and 31.63 mg/mL for BA and BAB, respectively. Boths extracts showed a DL<sub>50</sub> &gt; 2 g/kg and is classified as non-toxic. In Acetylated and Hydrolyzed extracts, acetyl-rutin and kaempferol, respectively were identified. Anti-inflammatory activity was assayed employing carrageenan model. The ED<sub>50</sub> values were &lt; 158.48 mg/kg by BA, BAB, Ac-BA, and H-BA, being more active the hydrolyzed extract (ED<sub>50</sub> = 63.09 and 64.56 mg/kg) for females and male mice. In topical TPA model, the ED<sub>50</sub> value were &lt; 0.47 mg/ear for BA, BAB, Ac-BA, and H-BA. Thermographic analysis is a non-invasive, cheap, effective and reproducible method to evaluate the anti-inflammatory effect of substances, since it quantifies the increase in temperature in an inflammatory process, so this technique constitutes an alternative in the search for this type of agent.</div></div>","PeriodicalId":8966,"journal":{"name":"Biomedicine & Pharmacotherapy","volume":"186 ","pages":"Article 117972"},"PeriodicalIF":6.9000,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Pharmacotherapy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0753332225001660","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

Abstract

Inflammation is a condition that affects a large percentage of the population, rendering the search for alternative treatments urgent. Medicinal plant such as Brugmansia arborea has been employed in traditional medicine, it is used to alleviate menstrual pain, acne, promote wound healing, and ease joint pain, headaches, and other ailments. Some alkaloids (mainly atropine, scopolamine, and nor-hyoscyamine) have been described. In this paper, the in-vitro cultivation from leaf explants of B. arborea through indirect organogenesis is described. From wild plants (BA) and shoots (BAB) their extracts were prepared, and were analyzed by thin-layer chromatography and gas chromatography-mass spectrometry, identifying 18 main metabolites. Antioxidant activity was assessed with EC50 = 199.52 and 31.63 mg/mL for BA and BAB, respectively. Boths extracts showed a DL50 > 2 g/kg and is classified as non-toxic. In Acetylated and Hydrolyzed extracts, acetyl-rutin and kaempferol, respectively were identified. Anti-inflammatory activity was assayed employing carrageenan model. The ED50 values were < 158.48 mg/kg by BA, BAB, Ac-BA, and H-BA, being more active the hydrolyzed extract (ED50 = 63.09 and 64.56 mg/kg) for females and male mice. In topical TPA model, the ED50 value were < 0.47 mg/ear for BA, BAB, Ac-BA, and H-BA. Thermographic analysis is a non-invasive, cheap, effective and reproducible method to evaluate the anti-inflammatory effect of substances, since it quantifies the increase in temperature in an inflammatory process, so this technique constitutes an alternative in the search for this type of agent.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
11.90
自引率
2.70%
发文量
1621
审稿时长
48 days
期刊介绍: Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.
期刊最新文献
A phthalimide-triazole derivative obtained by click chemistry exhibits trypanocidal activity, induces autophagy and ameliorates Trypanosoma cruzi infection Discovery of N-substituted-2-((arylethyl)amino)-2-(2-methoxyphenyl) acetamides: A novel family of antiplatelet agents Anti-inflammatory, antioxidant, and acute toxicity of Brugmansia arborea extracts from wild plants and shoots obtained by indirect organogenesis. A thermographic assay to anti-inflammatory evaluation Two concurrent mechanisms are responsible for the INa increase produced by dapagliflozin and empagliflozin in healthy and heart failure cardiomyocytes Discussion of the material basis for prevention and treatment of pulmonary fibrosis using naturally medicinal and edible homologous herbs based on the dynamic process of Nrf2, NF-κB and TGF-β in PF
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1