Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

IF 3.5 3区医学 Q3 IMMUNOLOGY Microbial pathogenesis Pub Date : 2025-06-01 Epub Date: 2025-03-17 DOI:10.1016/j.micpath.2025.107490

Heping Li , Danying Xie , Ruochao Huang , Bowen Shi , Jinghao Xie , Chen Yang , Xinyan Yao , Chaoxiang Jia , Guoyu Yang , Shuang Guo , Yueying Wang

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Abstract

Bacteriophage therapy has proved to be an attractive candidate for decreasing drug-resistant bacteria. In this study, a novel E. coli phage vB_EcoP_HC25 was isolated from sewage. Phage vB_EcoP_HC25 has an elongated head and a short tail, exhibiting the typical features of the rare C3 morphotype. The burst size was approximately 10⁵ PFU/cell. Phage vB_EcoP_HC25 exhibited good pH range (pH 5–11) and temperature tolerance (<60 °C). The genomic analysis revealed that phage vB_EcoP_HC25 does not carry any virulence factor genes or antibiotic resistance genes. Moreover, it was found that vB_EcoP_HC25 is a promising candidate phage for biocontrol against antibiotic-resistant E. coli in milk and chicken meat. In addition, the results of the mental state, behavior, serum inflammatory factors, and intestinal morphology analyses indicated that phage vB_EcoP_HC25 was effective to control colibacillosis in chicks infected with E. coli.

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噬菌体vB_EcoP_HC25的鉴定及其对鸡大肠杆菌病的治疗作用

噬菌体治疗已被证明是减少耐药细菌的一个有吸引力的候选者。本研究从污水中分离到了一种新的大肠杆菌噬菌体vB_EcoP_HC25。噬菌体vB_EcoP_HC25头长尾短，具有罕见的C3型噬菌体的典型特征。爆发大小约为105 PFU/细胞。噬菌体vB_EcoP_HC25具有良好的pH范围（pH 5 ~ 11）和耐温性（60℃）。基因组分析显示，噬菌体vB_EcoP_HC25不携带任何毒力因子基因或抗生素耐药基因。此外，发现vB_EcoP_HC25是一种很有前景的候选噬菌体，可用于对牛奶和鸡肉中的耐药大肠杆菌进行生物防治。此外，精神状态、行为、血清炎症因子和肠道形态分析结果表明，噬菌体vB_EcoP_HC25对大肠杆菌感染雏鸡大肠杆菌病的控制效果较好。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)