Amelioration of Glutamate-induced Toxicity by a New Thyrotropin-releasing Hormone (TRH) Analogue PYR-l-(2,5-Dibromo)-His-l-ProNH₂.

IF 2.4 Q4 NEUROSCIENCES Annals of Neurosciences Pub Date : 2025-03-13 DOI:10.1177/09727531241305505

Mallikarjuna R Sunkara, Jitendra N Singh, C L Meena, A B Pant, Rahul Jain, Shyam S Sharma

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Abstract

Background: Glutamate has been implicated in the pathophysiology of central nervous system diseases, including stroke.

Purpose: In this study, the neuroprotective potential of the newly synthesised thyrotropin-releasing hormone (TRH) analogue [Pyr-l-(2,5-dibromo)-His-l-ProNH₂; NP-2376] against glutamate-induced injury model were investigated.

Methods: Cortical neurons isolated from neonatal rats were used to evaluate the effects of the NP-2376. Cortical neurons were pre-treated with NP-2376 (6, 12 and 24 h) prior to glutamate (15 mM) exposure. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assay, and oxidative stress by chloromethyl-2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and glutathione assays.

Results: NP-2376 protected against glutamate-induced cortical neuron death and oxidative stress in a dose-dependent manner.

Conclusions: This study demonstrates the neuroprotective potential of TRH analogue NP-2376 against glutamate-induced toxicity, which is attributed to a decrease in oxidative stress.

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一种新的促甲状腺激素释放激素（TRH）类似物PYR-l-(2,5-二溴)-His-l-ProNH2可改善谷氨酸诱导的毒性。

背景：谷氨酸参与中枢神经系统疾病的病理生理，包括中风。目的：在本研究中，新合成的促甲状腺激素释放激素（TRH）类似物[pyr - 1 -（2,5-二溴）- his -l- pronh2；NP-2376]对谷氨酸损伤模型的影响。方法：采用新生大鼠皮质神经元，观察NP-2376的作用。在谷氨酸暴露（15 mM）之前，用NP-2376预处理皮质神经元（6、12和24小时）。采用3-（4,5-二甲基噻唑-2-酰基）-2,5-二苯基溴化四唑（MTT）和中性红色摄取（NRU）法测定细胞活力，采用氯甲基-2,7-二氯二氢荧光素双乙酸酯（DCFH-DA）和谷胱甘肽法测定氧化应激。结果：NP-2376对谷氨酸诱导的皮质神经元死亡和氧化应激具有剂量依赖性。结论：本研究表明TRH类似物NP-2376具有抗谷氨酸诱导毒性的神经保护作用，其机制可能与降低氧化应激有关。

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Annals of Neurosciences NEUROSCIENCES-

CiteScore

2.40

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0.00%

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