Impact of amino acid-regulated beta tricalcium phosphate hydroxyapatite on enamel: An updated perspective on hydroxyapatite crystal growth for adhesive bonding?

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of dentistry Pub Date : 2025-05-01 Epub Date: 2025-03-15 DOI:10.1016/j.jdent.2025.105693

Umer Daood , Sahar Fatima , Ng Mei Liit , Ilhaam Muneer Babar , Cynthia Yiu , Ove A. Peters , Jukka Matinlinna , Salvatore Sauro , Igor R Blum , Zeeshan Sheikh

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Abstract

Aims and Objectives

To analyse development of hydroxyapatite (HAp) crystals doped with glutamic acid and phosphorine residue (Ser-OPO₃) having self-remineralization, biocompatible and optimum bonding properties

Materials and Methods

β-tri calcium phosphate, ammonium phosphate dibasic HAp was dissolved with strontium Flouride Glu or Ser-OPO₃ components and utilised in two _0.1 _{% TCP}Ca₃(PO₄)_{2 Str/FL} and _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} groups. HAp formulation was evaluated for particle-size analysis, crystal size and indices using FTIR. Demineralized enamel specimens were treated and characterized using transmission electron microscopy (TEM), Xray diffraction (XRD), solid state NMR, and mechanical properties. MC3T3 mouse fibroblastic cells were evaluated for alkaline-phosphate activity (ALP). Human gingival fibroblasts (PGF) were evaluated for morphology. After modifying Universal adhesives, tensile force was assessed at 24 hr and 3 months.

Result

Increasing concentrations of experimental solution exposure significantly affect mean diameter of HAp. The increasing FTIR intensity, were displayed; _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} > _0.1 _{% TCP}Ca₃(PO₄)_{2 Str/FL}> control. _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} revealed existence of distinct crystals, with planes orientated perpendicular to longer axis of tooth. XRD pattern indicated crystal growth with a rise in peak intensities in _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} group. The NMR spectra for Glu and Ser-OPO₃ elucidated interactions between carbon atoms in amino acid and hydrogen atoms on HAp surfaces. Surface microhardness of _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} specimens showed significantly higher values on day 28 (p < 0.05). ALP density value of cells was significantly higher for _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} group on day 7 and 14. _0.1 _{% TCP}Ca₃(PO₄)_{2 Str/FL} and _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} enhanced migration of PGF cells in comparison to the control group (p < 0.05). _0.1 _{% TCP}Ca₃(PO₄)_{2 Str/FL} and _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} groups showed significant differences in bond strength at different time points

Conclusion

_0.1 _{% TCP}Ca₃(PO₄)_{2 Str/FL} and _0.2 _{% TCP}Ca₃(PO₄)_{2 Str/FL} were structurally integrated into the lattice demonstrating changes within the crystallite size and became conducive for adhesive bonding. Clinical Significance: The new amino acid-based hydroxyapatite formulation is chemically stable due to the substitution of OH- with F^- and is crucial in the rehardening of caries and adhesive bonding.

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氨基酸调控的β -磷酸三钙羟基磷灰石对牙釉质的影响：羟基磷灰石晶体生长的最新研究进展？

目的与目的：研究具有自矿化、生物相容性和最佳键合性能的谷氨酸磷渣（Ser-OPO3）掺杂羟基磷灰石（HAp）晶体的制备。材料与方法：将β-三磷酸钙、磷酸铵二盐型HAp与氟化锶Glu或Ser-OPO3组分溶解，分别制备0.1% TCPCa3(PO4)2 Str/FL和0.2% TCPCa3(PO4)2 Str/FL基团。用FTIR对HAp配方的粒度分析、晶体尺寸和指标进行了评价。用透射电镜（TEM）、x射线衍射（XRD）、固体核磁共振（NMR）和力学性能对脱矿牙釉质样品进行了处理和表征。测定MC3T3小鼠成纤维细胞的碱性-磷酸盐活性（ALP）。对人牙龈成纤维细胞（PGF）进行形态学评价。改良万能胶粘剂后，于24小时和3个月时评估拉伸力。结果：实验溶液暴露浓度的增加显著影响HAp的平均直径。FTIR强度逐渐增大；0.2% TCPCa3(PO4)2 Str/FL> 0.1% TCPCa3(PO4)2 Str/FL>控制。0.2% TCPCa3(PO4)2 Str/FL显示出明显的晶体，其平面垂直于齿长轴。XRD谱图显示，在0.2% TCPCa3(PO4)2 Str/FL基团中，晶体生长，峰强度上升。Glu和Ser-OPO3的核磁共振光谱揭示了氨基酸碳原子与HAp表面氢原子之间的相互作用。0.2% TCPCa3(PO4)2 Str/FL样品的表面显微硬度在第28天显著升高（p< 0.05）。0.2% TCPCa3(PO4)2 Str/FL组细胞ALP密度值在第7天和第14天显著升高。与对照组相比，0.1% TCPCa3(PO4)2 Str/FL和0.2% TCPCa3(PO4)2 Str/FL增强了PGF细胞的迁移能力(p0.1% TCPCa3（PO4)2 Str/FL和0.2% TCPCa3(PO4)2 Str/FL组在不同时间点的结合强度有显著差异）。结论：0.1% TCPCa3(PO4)2 Str/FL和0.2% TCPCa3(PO4)2 Str/FL在结构上整合到晶格中，显示出晶体大小的变化，有利于粘附结合。临床意义：新的氨基酸基羟基磷灰石配方由于羟基-被F-取代，化学性质稳定，对龋的再硬化和粘接至关重要。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.