{"title":"Protocol for capturing genome-wide chromatin interactions in Arabidopsis.","authors":"Jiayue Shu, Suxin Xiao, Dingyue Wang, Minqi Yang, Yue Zhou","doi":"10.1016/j.xpro.2025.103702","DOIUrl":null,"url":null,"abstract":"<p><p>High-order chromatin structure is essential for the regulation of plant growth and development. Here, we present a protocol for capturing genome-wide chromatin interactions in Arabidopsis. We describe steps for tissue fixation, nuclei isolation, chromatin digestion, ligation, sonication, and library preparation. This protocol has potential application in 3D chromatin analysis in many species of plants. For complete details on the use and execution of this protocol, please refer to Shu et al.,<sup>1</sup> Yin et al.,<sup>2</sup> and Yang et al.<sup>3</sup><sup>,</sup><sup>4</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 2","pages":"103702"},"PeriodicalIF":1.3000,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103702","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
High-order chromatin structure is essential for the regulation of plant growth and development. Here, we present a protocol for capturing genome-wide chromatin interactions in Arabidopsis. We describe steps for tissue fixation, nuclei isolation, chromatin digestion, ligation, sonication, and library preparation. This protocol has potential application in 3D chromatin analysis in many species of plants. For complete details on the use and execution of this protocol, please refer to Shu et al.,1 Yin et al.,2 and Yang et al.3,4.
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