Modifications in Leaf Anatomical Traits of Coffea spp. Genotypes Induced by Management × Season Interactions.

Abstract

Leaf anatomical traits are influenced by environmental and genetic factors; however, studies that investigate the genotype × environment interaction on these traits are scarce. This study hypothesized that (1) the leaf anatomy of Coffea spp. genotypes is varied, and (2) interactions between managements and seasons significantly influence leaf anatomical traits, inducing a clear adaptation to specific environments. Possible modifications of leaf anatomy in Coffea spp. genotypes were investigated under different managements: full-sun monoculture at low-altitude (MLA), full-sun monoculture at high altitude (MHA), and low-altitude agroforestry (AFS), in winter and summer. The genotype influenced all leaf anatomical traits investigated, contributing to 2.3-20.6% of variance. Genotype × environment interactions contributed to 2.3-95.8% of variance to key traits. The effects of genotype × management interactions were more intense than those of genotype × season interactions on traits such as leaf thickness, palisade parenchyma thickness, abaxial epidermis, and polar and equatorial diameter of the stomata. The management AFS was more effective in altering leaf anatomical traits than the altitude differences between MLA and MHA, regardless of the season. These findings provide valuable insights for future research and for the development of strategies to improve the adaptation of coffee plants to changing environmental conditions.