Ubiquitin Ligase Gene OsPUB57 Negatively Regulates Rice Blast Resistance.

IF 4.1 2区生物学 Q1 PLANT SCIENCES Plants-Basel Pub Date : 2025-03-01 DOI:10.3390/plants14050758

Jian Zhang, Qiang Du, Yugui Wu, Mengyu Shen, Furong Gao, Zhilong Wang, Xiuwen Xiao, Wenbang Tang, Qiuhong Chen

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Abstract

The ubiquitination and degradation of proteins are widely involved in plant biotic and abiotic stress responses. E3 ubiquitin ligases play an important role in the ubiquitination of specific proteins. In this study, we identified the function of a U-box E3 ubiquitin ligase gene OsPUB57 in rice. Expression analyses revealed that OsPUB57 was mainly expressed in the aboveground part of rice. Drought, salt, cold, JA (jasmonic acid), PAMPs (pathogen-associated molecular patterns) or Magnaportheoryzae treatment could significantly suppress the expression of OsPUB57 in rice. Compared with wild-type plants, OsPUB57-overexpressing plants showed a decrease in resistance to M. oryzae, while the mutant plants exhibited an enhancement of M. oryzae resistance. The expression level detection indicated that OsPUB57 negatively regulates rice blast resistance, probably by down-regulating the expression of the defense-related genes OsPR1a and OsAOS2. This study provides a candidate gene for the genetic improvement of rice blast resistance.

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泛素连接酶基因OsPUB57负调控水稻稻瘟病抗性

蛋白质的泛素化和降解广泛涉及植物的生物和非生物胁迫反应。E3 泛素连接酶在特定蛋白质的泛素化过程中发挥着重要作用。本研究确定了水稻中 U-box E3 泛素连接酶基因 OsPUB57 的功能。表达分析表明，OsPUB57 主要在水稻的地上部分表达。干旱、盐渍、低温、JA（茉莉酸）、PAMPs（病原相关分子模式）或木兰花处理均能显著抑制水稻中OsPUB57的表达。与野生型植株相比，OsPUB57高表达植株对M. oryzae的抗性下降，而突变植株对M. oryzae的抗性增强。表达水平的检测结果表明，OsPUB57可能通过下调防御相关基因OsPR1a和OsAOS2的表达来负向调控稻瘟病的抗性。本研究为遗传改良稻瘟病抗性提供了一个候选基因。

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来源期刊

Plants-Basel Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

6.50

自引率

11.10%

发文量

2923

审稿时长

15.4 days

期刊介绍： Plants (ISSN 2223-7747), is an international and multidisciplinary scientific open access journal that covers all key areas of plant science. It publishes review articles, regular research articles, communications, and short notes in the fields of structural, functional and experimental botany. In addition to fundamental disciplines such as morphology, systematics, physiology and ecology of plants, the journal welcomes all types of articles in the field of applied plant science.