Compound-Specific Carbon and Nitrogen Isotopic Analyses of Underivatized Pyrimidine and Purine Nucleobases

IF 2.9 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY ACS Earth and Space Chemistry Pub Date : 2025-03-03 DOI:10.1021/acsearthspacechem.4c00110

Toshiki Koga*, Yoshinori Takano, Nanako O. Ogawa, Emily H. Hollingsworth, Yasuhiro Oba and Naohiko Ohkouchi,

{"title":"Compound-Specific Carbon and Nitrogen Isotopic Analyses of Underivatized Pyrimidine and Purine Nucleobases","authors":"Toshiki Koga*, Yoshinori Takano, Nanako O. Ogawa, Emily H. Hollingsworth, Yasuhiro Oba and Naohiko Ohkouchi, ","doi":"10.1021/acsearthspacechem.4c00110","DOIUrl":null,"url":null,"abstract":"We report on a newly developed method for compound-specific isotopic analysis (CSIA) of carbon (δ13C) and nitrogen (δ15N) in underivatized pyrimidine (cytosine, uracil, and thymine) and purine (adenine, guanine, hypoxanthine, and xanthine) nucleobases. The nucleobases are isolated by multistep purification using high-performance liquid chromatography (HPLC). HPLC separation and its wet chemical pretreatments were optimized to isolate these nucleobases without the need for chemical derivatization. Subsequently, the carbon and nitrogen isotopic compositions of these underivatized nucleobases were analyzed by nanoscale elemental analysis/isotope-ratio mass spectrometry (nano-EA/IRMS). The δ13C and δ15N measurements of isolated standard nucleobases indicated minimal isotopic fractionation during the two-step HPLC purification. This HPLC × nano-EA/IMS methodology was utilized to determine the isotopic composition of nucleobases in spinach (δ13C: −27.0 to −19.8‰, δ15N: +2.8 to +6.7‰) and coastal sediment (δ13C: −14.9 to −9.2 ‰, δ15N: +4.5 to +9.0‰) samples. The CSIA of nucleobases can provide insight into how living organisms modulate their biochemical pathways in response to environmental and metabolic variations.","PeriodicalId":15,"journal":{"name":"ACS Earth and Space Chemistry","volume":"9 3","pages":"424–432 424–432"},"PeriodicalIF":2.9000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Earth and Space Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsearthspacechem.4c00110","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

We report on a newly developed method for compound-specific isotopic analysis (CSIA) of carbon (δ¹³C) and nitrogen (δ¹⁵N) in underivatized pyrimidine (cytosine, uracil, and thymine) and purine (adenine, guanine, hypoxanthine, and xanthine) nucleobases. The nucleobases are isolated by multistep purification using high-performance liquid chromatography (HPLC). HPLC separation and its wet chemical pretreatments were optimized to isolate these nucleobases without the need for chemical derivatization. Subsequently, the carbon and nitrogen isotopic compositions of these underivatized nucleobases were analyzed by nanoscale elemental analysis/isotope-ratio mass spectrometry (nano-EA/IRMS). The δ¹³C and δ¹⁵N measurements of isolated standard nucleobases indicated minimal isotopic fractionation during the two-step HPLC purification. This HPLC × nano-EA/IMS methodology was utilized to determine the isotopic composition of nucleobases in spinach (δ¹³C: −27.0 to −19.8‰, δ¹⁵N: +2.8 to +6.7‰) and coastal sediment (δ¹³C: −14.9 to −9.2 ‰, δ¹⁵N: +4.5 to +9.0‰) samples. The CSIA of nucleobases can provide insight into how living organisms modulate their biochemical pathways in response to environmental and metabolic variations.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

未电离嘧啶和嘌呤核碱基的化合物特异性碳氮同位素分析

我们报告了一种新开发的化合物特异性同位素分析（CSIA）方法，用于分析未充分活化的嘧啶（胞嘧啶、尿嘧啶和胸腺嘧啶）和嘌呤（腺嘌呤、鸟嘌呤、次黄嘌呤和黄嘌呤）核碱基中的碳（δ13C）和氮（δ15N）。核碱基通过使用高效液相色谱法（HPLC）进行多步纯化来分离。对高效液相色谱分离及其湿化学预处理进行了优化，以在无需化学衍生的情况下分离出这些核碱基。随后，通过纳米级元素分析/同位素比质谱（nano-EA/IRMS）分析了这些未充分活化的核碱基的碳和氮同位素组成。对分离的标准核碱基进行的δ13C和δ15N测量表明，在两步高效液相色谱纯化过程中同位素分馏极小。利用这种 HPLC × 纳米EA/IMS 方法测定了菠菜（δ13C：-27.0 至 -19.8‰，δ15N：+2.8 至 +6.7‰）和沿海沉积物（δ13C：-14.9 至 -9.2‰，δ15N：+4.5 至 +9.0‰）样品中核酸碱基的同位素组成。核碱基的 CSIA 可以让人们深入了解生物体是如何根据环境和新陈代谢的变化来调整其生化途径的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

ACS Earth and Space Chemistry Earth and Planetary Sciences-Geochemistry and Petrology

CiteScore

5.30

自引率

11.80%

发文量

249

期刊介绍： The scope of ACS Earth and Space Chemistry includes the application of analytical, experimental and theoretical chemistry to investigate research questions relevant to the Earth and Space. The journal encompasses the highly interdisciplinary nature of research in this area, while emphasizing chemistry and chemical research tools as the unifying theme. The journal publishes broadly in the domains of high- and low-temperature geochemistry, atmospheric chemistry, marine chemistry, planetary chemistry, astrochemistry, and analytical geochemistry. ACS Earth and Space Chemistry publishes Articles, Letters, Reviews, and Features to provide flexible formats to readily communicate all aspects of research in these fields.

期刊最新文献

Issue Editorial Masthead Issue Publication Information IR-Action Spectroscopy of the Astrochemically Relevant HCCS+ Cation Ab Initio Molecular Dynamics Study of H2SO3 Ionization in Hydrothermal Fluids and Nanodroplets CO Diffusion on Interstellar Amorphous Solid Water: A Computational Study