{"title":"MicroRNA-mediated regulation in anoxic Lithobates sylvaticus liver","authors":"Yasser Attaie, Kenneth B. Storey","doi":"10.1016/j.cbd.2025.101487","DOIUrl":null,"url":null,"abstract":"<div><div>The wood frog, <em>Lithobates sylvaticus</em> (formerly <em>Rana sylvatica</em>), endures various physiological stressors including freezing, anoxia, dehydration, and hyperglycemia during winter, relying on metabolic stress response to survive. microRNA (miRNA)-mediated gene regulation is a well-documented stress response, however, its role during anoxia in <em>L. sylvaticus</em> liver remains underexplored. This research identifies seven miRNAs with significant differential expression during anoxia. Bioinformatic analyses predicted downregulation of monounsaturated fatty acid biosynthesis and membrane fluidity pathways, alongside upregulation of insulin signalling pathways (PI3K/Akt, mTOR). These results suggest that miRNAs contribute to gene regulation in L. <em>sylvaticus</em> during anoxia.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101487"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1744117X25000759","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The wood frog, Lithobates sylvaticus (formerly Rana sylvatica), endures various physiological stressors including freezing, anoxia, dehydration, and hyperglycemia during winter, relying on metabolic stress response to survive. microRNA (miRNA)-mediated gene regulation is a well-documented stress response, however, its role during anoxia in L. sylvaticus liver remains underexplored. This research identifies seven miRNAs with significant differential expression during anoxia. Bioinformatic analyses predicted downregulation of monounsaturated fatty acid biosynthesis and membrane fluidity pathways, alongside upregulation of insulin signalling pathways (PI3K/Akt, mTOR). These results suggest that miRNAs contribute to gene regulation in L. sylvaticus during anoxia.
期刊介绍：
Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology.
Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.
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