Role of ERG3 mutation and expression in azole resistant Candida albicans isolated from vulvovaginal candidiasis patients.

IF 1.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Pakistan Journal of Medical Sciences Pub Date : 2025-03-01 DOI:10.12669/pjms.41.3.9325

Ronaq Zaman, Ihsan Ullah, Ambreen Arif

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Abstract

Objective: The study aimed to investigate mutations and mRNA expression of the ERG3 gene in resistant Candida albicans isolates.

Methods: This cross-sectional study was conducted from October 2018 to June 2019. High vaginal swab samples were collected from Hayatabad Medical Complex and transported to Khyber Medical University. Samples were inoculated on different media and identified by 20C AUX strips. Antifungal susceptibility was determined using the disc diffusion and broth microdilution methods. The ERG3 gene was amplified and sequenced to find amino acid polymorphisms. Real-time PCR was performed to study level of ERG3 expression.

Results: A total of seventy-three (n=73) Candida albicans out of 369 samples were isolated. Among the isolates 49.3%, 54.8%, 53.4%, 47.9%, 30.1% were resistant to fluconazole, Clotrimazole, Miconazole, Voriconazole and Itraconazole, respectively. Sanger sequencing of ERG3 gene of isolates revealed six synonymous mutations. Expression level of mRNA of ERG3 gene in azoles sensitive stains (3.72±2.22) was higher than those in the resistant Candida albican strains (1.74±0.96).

Conclusion: This study revealed synonymous mutations and low expression of ERG3 gene in azole-resistant C. albican.

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ERG3突变及表达在外阴阴道念珠菌病患者耐唑白色念珠菌中的作用

目的：研究耐药白色念珠菌分离株中ERG3基因的突变及其mRNA表达。方法：本横断面研究于2018年10月至2019年6月进行。从哈亚塔巴德医疗中心收集了大量阴道拭子样本，并运往开伯尔医科大学。样品接种于不同培养基上，用20C AUX试纸进行鉴定。采用圆盘扩散法和肉汤微量稀释法测定其抗真菌敏感性。对ERG3基因进行扩增和测序，发现其氨基酸多态性。采用Real-time PCR检测ERG3的表达水平。结果：369份标本中共分离出白色念珠菌73株（n=73）。对氟康唑、克曲霉唑、咪康唑、伏立康唑和伊曲康唑耐药的分别为49.3%、54.8%、53.4%、47.9%和30.1%。分离株ERG3基因Sanger测序显示6个同义突变。氮唑敏感菌株中ERG3基因mRNA表达量（3.72±2.22）高于耐药菌株（1.74±0.96）。结论：本研究揭示了耐唑白色念珠菌的同义突变和ERG3基因的低表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pakistan Journal of Medical Sciences 医学-医学：内科

CiteScore

4.10

自引率

9.10%

发文量

363

审稿时长

3-6 weeks

期刊介绍： It is a peer reviewed medical journal published regularly since 1984. It was previously known as quarterly "SPECIALIST" till December 31st 1999. It publishes original research articles, review articles, current practices, short communications & case reports. It attracts manuscripts not only from within Pakistan but also from over fifty countries from abroad. Copies of PJMS are sent to all the import medical libraries all over Pakistan and overseas particularly in South East Asia and Asia Pacific besides WHO EMRO Region countries. Eminent members of the medical profession at home and abroad regularly contribute their write-ups, manuscripts in our publications. We pursue an independent editorial policy, which allows an opportunity to the healthcare professionals to express their views without any fear or favour. That is why many opinion makers among the medical and pharmaceutical profession use this publication to communicate their viewpoint.