Influence of the lncRNA SLC9A3-AS1 on colon cancer and the biological activities of colon cancer cells.

IF 2.9 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Discover. Oncology Pub Date : 2025-03-19 DOI:10.1007/s12672-025-02134-w

Xiulian Xu, Hongyi Qing, Chunyan Jiang, Xiaofeng Zhao, Jinlai Wei

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Abstract

Background: Circulating long non-coding RNAs expression was associated with diagnosis and therapies of various diseases. The current study investigated the expression of lncRNA SLC9A3-AS1 in the serum samples from colon cancer patients and explored its potential functions in colon cancer cells.

Methods: Serum expression levels of SLC9A3-AS1 and miR-486 were measured in 130 patients with colon cancer and 96 healthy individuals using RT-qPCR. The influence of SLC9A3-AS1 expression and miR-486 expression on colon cancer cellular behaviors was detected by MTT assay and Transwell chamber assays. Pearson correlation analysis was used to analyze the association between SLC9A3-AS1 and miR-486.

Results: We found serum expression levels of SLC9A3-AS1 were overexpressed in sera of colon cancer patients. ROC curve analysis showed that SLC9A3-AS1 had a high area under the ROC curve value for early detection of colon cancer patients from a healthy control. The proliferation potential, migration, and invasion behaviors were weakened by si-SLC9A3-AS1 and reversed by the miR-486 inhibitor.

Conclusion: Serum SLC9A3-AS1 may be used as a non-invasive diagnostic predictor for the early screening of colon cancer. LncRNA SLC9A3-AS1 affects colon cancer cellular activities by negatively modulating miR-486. A major limitation of this study is the small sample size, and in addition, the lack of longitudinal data prevented us from conducting an in-depth analysis of long-term changes in variables.

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lncRNA SLC9A3-AS1对结肠癌及结肠癌细胞生物学活性的影响

背景：循环长链非编码rna的表达与多种疾病的诊断和治疗有关。本研究通过研究lncRNA SLC9A3-AS1在结肠癌患者血清样本中的表达，探索其在结肠癌细胞中的潜在功能。方法：采用RT-qPCR检测130例结肠癌患者和96例健康人血清中SLC9A3-AS1和miR-486的表达水平。MTT法和Transwell室法检测SLC9A3-AS1表达和miR-486表达对结肠癌细胞行为的影响。采用Pearson相关分析分析SLC9A3-AS1与miR-486的相关性。结果：我们发现SLC9A3-AS1在结肠癌患者血清中存在过表达。ROC曲线分析显示，SLC9A3-AS1对健康对照早期发现结肠癌患者的ROC曲线值下面积较高。si-SLC9A3-AS1可减弱细胞的增殖潜能、迁移和侵袭行为，miR-486抑制剂可逆转细胞的增殖潜能。结论：血清SLC9A3-AS1可作为结肠癌早期筛查的无创诊断指标。LncRNA SLC9A3-AS1通过负性调节miR-486影响结肠癌细胞活性。本研究的一个主要限制是样本量小，此外，缺乏纵向数据使我们无法对变量的长期变化进行深入分析。

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