{"title":"Deep Learning-Driven Computational Approaches for Studying Intrinsically Disordered Regions in S100-A9.","authors":"Gionathan L Distefano, Fabio D'Amico","doi":"10.1007/7651_2025_617","DOIUrl":null,"url":null,"abstract":"<p><p>Intrinsically disordered regions (IDRs) are flexible protein regions and lack a fixed three-dimensional structure, which makes them difficult to study using traditional structural methods. However, artificial intelligence can be helpful in predicting, analyzing, and modeling these regions. This chapter provides a simple protocol for a preliminary-level approach to identifying protein IDRs. By reporting on the S100-A9 protein as a case study example, characterization of the IDRs of this molecule could provide further details on the complex molecular interactions involved in psoriasis, particularly those related to inflammation, immune dysregulation, and keratinocyte behavior.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/7651_2025_617","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Intrinsically disordered regions (IDRs) are flexible protein regions and lack a fixed three-dimensional structure, which makes them difficult to study using traditional structural methods. However, artificial intelligence can be helpful in predicting, analyzing, and modeling these regions. This chapter provides a simple protocol for a preliminary-level approach to identifying protein IDRs. By reporting on the S100-A9 protein as a case study example, characterization of the IDRs of this molecule could provide further details on the complex molecular interactions involved in psoriasis, particularly those related to inflammation, immune dysregulation, and keratinocyte behavior.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
