Chemical Composition of Secretions from Facial Glands of Captive Hoary Bat (Lasiurus cinereus) in Virginia.

Abstract

Our study represents the first known report of hoary bat (Lasiurus cinereus) facial gland histology and chemical characterization of facial gland secretions. While in captivity from October 2023 to February 2024, facial secretions of a male hoary bat were observed and collected. Gas chromatography-mass spectrometry (GC-MS) analysis of a methanol extraction of November 2023 samples identified 23 compounds after comparing retention times, mass spectra, and Kovats retention index. These compounds consisted of alkanes, alcohols, ketones, terpenoids, monoacylglycerols, and androgens. Of interest was the identification of five androstane derivatives, testosterone metabolites, that have not been seen previously in mammal secretions and indicate possible mating readiness. The chemical analyses from November swabs and histological examination of facial skin in February suggest that this male was actively secreting mate-signaling compounds throughout late fall and winter months. Anecdotal mating records on wintering grounds combined with this bat's warm captive environment likely allowed for this extended mating readiness for his many months at the wildlife center.