NanoASV: a snakemake workflow for reproducible field-based Nanopore full-length 16S metabarcoding amplicon data analysis.

Arthur Cousson, Frédéric Mahé, Ulysse Guyet, Damase Razafimahafaly, Laetitia Bernard
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Abstract

Summary: NanoASV is a conda environment and snakemake-based workflow using state-of-the-art bioinformatics software to process full-length SSU rRNA (16S/18S) amplicons acquired with Oxford Nanopore Sequencing technology. Its strength lies in reproducibility, portability, and the possibility to run offline, allowing in-field analysis. It can be installed on the Nanopore MK1C sequencing device and process data locally.

Availability and implementation: Source code and documentation are freely available at https://github.com/ImagoXV/NanoASV and Zenodo archive at https://doi.org/10.5281/zenodo.14730742.

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NanoASV:可重复的基于现场的纳米孔全长16S元条形码扩增子数据分析的蛇形工作流程。
NanoASV是一个conda环境和基于蛇制的工作流程,使用最先进的生物信息学软件来处理全长SSU rRNA (16S/18S)扩增子,这些扩增子是通过牛津纳米孔测序技术获得的。它的优势在于再现性、便携性和离线运行的可能性,允许现场分析。它可以安装在Nanopore MK1C测序设备上,并在本地处理数据。可用性:源代码和文档可在https://github.com/ImagoXV/NanoASV免费获得- Zenodo存档https://doi.org/10.5281/zenodo.14730742.Supplementary信息:补充数据可在Bioinformatics在线获得。
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