Performance of microscopy and rapid test for the diagnosis of mixed malaria in an endemic area of Colombia.

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES Diagnostic microbiology and infectious disease Pub Date : 2025-03-12 DOI:10.1016/j.diagmicrobio.2025.116799

Alexandra Rios-Orrego, Tatiana M Lopera-Mesa, Veronica Sierra Cifuentes, Lina Zuluaga-Idárraga

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Abstract

Background: Mixed infection by Plasmodium (MIP) occurs when more than one species are infecting one individual at the same time.

Methods: Frequency of MIP was determined in a cohort of patients with acute febrile syndrome. Diagnostic performance of microscopy (MI) and a rapid diagnostic test (RDT) was evaluated against real-time PCR (qPCR), as reference method.

Results: The frequency of MIP was 3% by qPCR, 1.4% by MI and 0.4% by RDT. The sensitivity of MI and RDT for detecting MIP was 21.43% and 15.25%, respectively, and specificity was over 99% for both techniques.

Conclusion: The currently available tests for the diagnosis of Plasmodium in the field, showed poor performance for detecting MIP in this study. It is necessary to improve diagnostic tests sensitivity for the correct detection of these coinfections and to monitor the elimination of parasitemia to detect possible recurrences due to inadequate treatments.

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背景：疟原虫混合感染（MIP疟原虫混合感染（MIP）是指一种以上的疟原虫同时感染一个人：方法：在一组急性发热综合征患者中确定疟原虫混合感染的频率。结果：显微镜检查（MI）和快速诊断检测（RDT）的诊断性能与作为参考方法的实时 PCR（qPCR）进行了评估：结果：采用 qPCR 检测的 MIP 发生率为 3%，MI 为 1.4%，RDT 为 0.4%。MI 和 RDT 检测 MIP 的灵敏度分别为 21.43% 和 15.25%，两种技术的特异性均超过 99%：结论：在这项研究中，目前可用于现场诊断疟原虫的检测方法在检测 MIP 方面表现不佳。有必要提高诊断检测的灵敏度，以正确检测这些合并感染，并监测寄生虫血症的消除情况，以发现因治疗不当而可能导致的复发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.