Integrating Metabolic RNA Labeling-Based Time-Resolved Single-Cell RNA Sequencing with Spatial Transcriptomics for Spatiotemporal Transcriptomic Analysis (Small Methods 3/2025)

IF 9.1 2区 材料科学 Q1 CHEMISTRY, PHYSICAL Small Methods Pub Date : 2025-03-20 DOI:10.1002/smtd.202570019
Xiaoyong Chen, Shichao Lin, Honghai You, Jinyuan Chen, Qiaoyi Wu, Kun Yin, Fanghe Lin, Yingkun Zhang, Jia Song, Chenyu Ding, Dezhi Kang, Chaoyong Yang
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Abstract

Inside Back Cover

In article number 2401297, Ding, Kang, Yang, and co-workers integrate metabolic RNA labeling-based time-resolved scRNA-seq with spatial transcriptomics (10x Visium and in situ sequencing) to profile the transcriptional dynamics of glioblastoma cells. The spatiotemporal analysis elucidates the complex gene regulatory mechanism of EZH2-mediated mesenchymal transition in the tumor microenvironment.

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整合基于代谢RNA标记的时间分辨单细胞RNA测序与空间转录组学进行时空转录组学分析(Small Methods 3/2025)
在文章编号2401297中,Ding, Kang, Yang等人将基于代谢RNA标记的时间分辨scRNA-seq与空间转录组学(10x Visium和原位测序)结合起来,分析了胶质母细胞瘤细胞的转录动力学。时空分析阐明了ezh2在肿瘤微环境中介导间质转化的复杂基因调控机制。
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来源期刊
Small Methods
Small Methods Materials Science-General Materials Science
CiteScore
17.40
自引率
1.60%
发文量
347
期刊介绍: Small Methods is a multidisciplinary journal that publishes groundbreaking research on methods relevant to nano- and microscale research. It welcomes contributions from the fields of materials science, biomedical science, chemistry, and physics, showcasing the latest advancements in experimental techniques. With a notable 2022 Impact Factor of 12.4 (Journal Citation Reports, Clarivate Analytics, 2023), Small Methods is recognized for its significant impact on the scientific community. The online ISSN for Small Methods is 2366-9608.
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