A laboratory-friendly protocol for freeze-drying sample preparation in ToF-SIMS single-cell imaging.

IF 4.2 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Frontiers in Chemistry Pub Date : 2025-03-07 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1523712

Xiujuan Shi, Mingru Liu, Yue Qi, Hongzhe Ma, Zhaoying Wang, Yanhua Chen, Zeper Abliz

引用次数: 0

Abstract

ToF-SIMS is a high spatial resolution imaging technique for cellular or subcellular analysis of biological samples. Accurate molecular data in single-cell studies depend on proper cell morphology and chemical integrity, highlighting the importance of sample preparation. In this work, we standardized a more efficient freeze-drying method using standard lab materials and improved the sample preparation process. Our comprehensive freeze-drying protocol for cellular samples, encompassing washing, fixation, and drying steps, facilitates the acquisition of enhanced cellular information and ensures high reproducibility. These improvements are poised to significantly advance single-cell mass spectrometry imaging research.

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用于ToF-SIMS单细胞成像的冷冻干燥样品制备的实验室友好协议。

ToF-SIMS是一种用于生物样品细胞或亚细胞分析的高空间分辨率成像技术。准确的分子数据在单细胞研究依赖于适当的细胞形态和化学完整性，强调样品制备的重要性。在这项工作中，我们标准化了一种使用标准实验室材料的更有效的冷冻干燥方法，并改进了样品制备工艺。我们对细胞样品的全面冷冻干燥方案，包括洗涤，固定和干燥步骤，有助于获得增强的细胞信息，并确保高再现性。这些改进有望显著推进单细胞质谱成像研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.