Metabolic profiling in experimental guinea pig models of bacterial and allergic inflammation.

IF 3.3 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Metabolomics Pub Date : 2025-03-23 DOI:10.1007/s11306-025-02239-x

J Hanusrichterova, E Baranovicova, R Barosova, M Kolomaznik, P Mikolka, P Kosutova, D Mokra, J Mokry, A Calkovska

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Abstract

Introduction: Based on distinct triggers, bacterial and allergen-induced inflammatory reactions have different pathophysiology. Metabolomic analysis is high-throughput technique that can provide potential biomarkers to distinguish between these responses.

Objectives: In order to find out the metabolic profiles of two types of inflammation, metabolites were analysed in blood plasma and bronchoalveolar lavage fluid (BALF) of guinea pigs subjected to bacterial lipopolysaccharide (LPS) or allergen ovalbumin (OVA).

Methods: Hydrogen-1 nuclear magnetic resonance (¹H NMR) spectroscopy for metabolite analysis was performed in samples of blood plasma and BALF of guinea pigs.

Results: Random forest algorithm built on combination of levels of circulating and BALF metabolites resulted in almost ideal discrimination between acute allergic and bacterial inflammation. The differences between inflammation triggered by LPS and OVA were manifested in shift in energy metabolism, metabolism of branched-chain amino acids (BCAAs)/branched-chain keto acids (BCKAs) with alterations in alanine and glutamine, which are linked with both, ammonia homeostasis as well as gluconeogenesis.

Conclusion: Distinct molecule nutrients are to be utilized during acute bacterial and allergic inflammatory response.

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细菌性和过敏性炎症实验豚鼠模型的代谢谱分析。

基于不同的触发因素，细菌和过敏原诱导的炎症反应具有不同的病理生理学。代谢组学分析是一种高通量技术，可以提供潜在的生物标志物来区分这些反应。目的：分析细菌脂多糖（LPS）和过敏原卵清蛋白（OVA）作用下豚鼠血浆和支气管肺泡灌洗液（BALF）的代谢物，以了解两种炎症的代谢谱。方法：采用氢-1核磁共振（1H NMR）对豚鼠血浆和BALF样品进行代谢物分析。结果：结合循环和BALF代谢物水平建立的随机森林算法对急性过敏性炎症和细菌性炎症的区分几乎是理想的。LPS和OVA引起的炎症的差异表现在能量代谢的改变、支链氨基酸(BCAAs)/支链酮酸（BCKAs）的代谢以及与两者相关的丙氨酸和谷氨酰胺的改变、氨稳态和糖异生。结论：在急性细菌性和变应性炎症反应中应使用不同的分子营养素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolomics 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.80%

发文量

审稿时长

2 months

期刊介绍： Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.