Xiaoya Su, Junyue He, Yunlong Liu, Keyu Liu, Bo Lei, Yi Zhong
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引用次数: 0
Abstract
Home-related cues, such as home bedding, influence affective behaviors in mice, including fear relief, anxiety reduction, and defensive aggression. Here, we present a protocol for labeling and manipulating "home"-activated neurons in the lateral hypothalamus (LH) using a Tet-Off inducible system. We describe steps for virus injection, postoperative recovery, and combining c-Fos-driven activity labeling with chemogenetics. This protocol integrates neuronal targeting with behavioral assays to provide insights into the functional role of "home"-activated neurons in home-driven behavioral adaptations. For complete details on the use and execution of this protocol, please refer to Su et al.1.
与家有关的线索，如家里的床上用品，会影响老鼠的情感行为，包括恐惧缓解、焦虑减少和防御性攻击。在这里，我们提出了一种使用Tet-Off诱导系统标记和操纵外侧下丘脑（LH）“家”激活神经元的方案。我们描述了病毒注射、术后恢复的步骤，以及将c- fos驱动的活性标记与化学遗传学相结合。该方案将神经元靶向与行为分析相结合，以深入了解“家庭”激活神经元在家庭驱动行为适应中的功能作用。有关本协议使用和执行的完整细节，请参见Su et al.1。
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