Role of octamer transcription factor 4 in proliferation, migration, drug sensitivity, and stemness maintenance of pancreatic cancer cells.

IF 3.2 Q3 ONCOLOGY World journal of clinical oncology Pub Date : 2025-03-24 DOI:10.5306/wjco.v16.i3.100723

Xue-Ying Shi, Xi-Lan Wang, Jin Zhao, Shi-Hai Yang, Cheng-Hai Zhang

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Abstract

Background: Pancreatic cancer (PC) is one of the most aggressive malignancies characterized by rapid progression and poor prognosis. The involvement of cancer stem cells (CSCs) and Octamer transcription factor 4 (OCT4) in PC pathobiology is being increasingly recognized.

Aim: To investigate the role of OCT4 in pancreatic CSCs and its effect on PC cell proliferation, migration, drug sensitivity, and stemness maintenance.

Methods: We analyzed OCT4 and CD133 expression in PC tissues and cell lines. BxPC-3 cells were used to assess the effects of OCT4 modulation on cellular behavior. Proliferation, migration, and stemness of BxPC-3 cells were evaluated, and the PI3K/AKT/mTOR pathway was examined to gain mechanistic insights.

Results: OCT4 and CD133 were significantly overexpressed in PC tissues. OCT4 modulation altered BxPC-3 cell proliferation, invasion, and stemness, with OCT4 overexpression (OV-OCT4) enhancing these properties and OCT4 interference decreasing them. OV-OCT4 activated the PI3K/AKT/mTOR pathway, which correlated with an increase in PC stem cells (PCSC).

Conclusion: OCT4 plays a crucial role in PCSCs by influencing the aggressiveness and drug resistance of PC cells, thus presenting itself as a potential therapeutic target.

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八聚体转录因子 4 在胰腺癌细胞的增殖、迁移、药物敏感性和干性维持中的作用。

背景：胰腺癌（PC）是最具侵袭性的恶性肿瘤之一，其特点是进展迅速，预后差。肿瘤干细胞（CSCs）和八聚体转录因子4 （OCT4）在PC病理生物学中的作用越来越被认识到。目的：探讨OCT4在胰腺CSCs中的作用及其对PC细胞增殖、迁移、药物敏感性和干细胞维持的影响。方法：分析OCT4和CD133在PC组织和细胞系中的表达。用BxPC-3细胞评价OCT4调控对细胞行为的影响。我们对BxPC-3细胞的增殖、迁移和干性进行了评估，并对PI3K/AKT/mTOR通路进行了研究，以了解其机制。结果：OCT4和CD133在PC组织中显著过表达。OCT4调控改变了BxPC-3细胞的增殖、侵袭和干细胞性，OCT4过表达（OV-OCT4）增强了这些特性，而OCT4干扰降低了这些特性。OV-OCT4激活PI3K/AKT/mTOR通路，这与PC干细胞（PCSC）的增加相关。结论：OCT4通过影响PCSCs的侵袭性和耐药，在PCSCs中发挥重要作用，是一个潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of clinical oncology ONCOLOGY-

自引率

0.00%

发文量

585

期刊介绍： The WJCO is a high-quality, peer reviewed, open-access journal. The primary task of WJCO is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of oncology. In order to promote productive academic communication, the peer review process for the WJCO is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJCO are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in oncology. Scope: Art of Oncology, Biology of Neoplasia, Breast Cancer, Cancer Prevention and Control, Cancer-Related Complications, Diagnosis in Oncology, Gastrointestinal Cancer, Genetic Testing For Cancer, Gynecologic Cancer, Head and Neck Cancer, Hematologic Malignancy, Lung Cancer, Melanoma, Molecular Oncology, Neurooncology, Palliative and Supportive Care, Pediatric Oncology, Surgical Oncology, Translational Oncology, and Urologic Oncology.