Conjugation of flaxseed protein and plant polysaccharides: Process optimization, structural characterization and technical-function evaluation

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED Food Hydrocolloids Pub Date : 2025-03-20 DOI:10.1016/j.foodhyd.2025.111371

Ziqin Ye , Shengrui Gan , Jinying Wang, Furong Yang, Guoxin Dong

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Abstract

In this study, the covalent conjugation conditions of flaxseed protein isolate (FPI) with flaxseed gum (FG), oat β-glucan (BG), and soybean polysaccharide (SP) were optimized separately using the ultrasound-assisted (U) method. The effects of the conjugation on the structure, function, and antioxidant properties of FPI were explored based on the optimal conditions. The results showed that the infrared absorption peaks of FPI in each sample changed and shifted in amide I and II bands after conjugation, indicating the progression of the Maillard reaction. Fluorescence intensity decreased and red-shifted emission peaks (10–15 nm), suggesting increased protein unfolding and improved hydrophilic interactions. Moreover, conjugation decreased the intensity of the UV absorption peaks, with the maximum absorption shifting to shorter wavelengths. Further validated the structural modifications associated with the Maillard reaction. Ultrasound-assisted conjugation also improved the functional properties in all samples, especially FPI-FG-U showed the best foaming capacity (improved to 80.39 %) and emulsification activity and stability (increased to 64.95 m²/g and 51.80 %), confirmed the correlation between structural changes and functional improvements. ABTS⁺ and DPPH radical scavenging experiments showed that the antioxidant capacity of FPI was enhanced by covalent conjugation. This study shows that the functional performance of FPI can be extended by covalently linking a combination of sonication, thus broadening their potential uses in the food sector and beyond.

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亚麻籽蛋白与植物多糖的偶联：工艺优化、结构表征和技术功能评价

本研究采用超声辅助(U)法分别优化了亚麻籽分离蛋白（FPI）与亚麻籽胶（FG）、燕麦β-葡聚糖（BG）和大豆多糖（SP）的共价偶联条件。以最佳条件为基础，探讨了共轭物对FPI结构、功能和抗氧化性能的影响。结果表明，共轭后各样品中FPI的红外吸收峰在酰胺I和酰胺II波段发生变化和位移，表明美拉德反应的进行。荧光强度下降，发射峰红移（10-15 nm），表明蛋白质展开增加，亲水性相互作用改善。此外，共轭作用降低了紫外吸收峰的强度，最大吸收峰向短波长偏移。进一步验证了与美拉德反应相关的结构修饰。超声辅助偶联也改善了所有样品的功能性能，特别是FPI-FG-U表现出最好的发泡能力（提高到80.39%）和乳化活性和稳定性（提高到64.95 m2/g和51.80%），证实了结构变化与功能改善之间的相关性。通过ABTS+和DPPH自由基清除实验表明，FPI的抗氧化能力通过共价偶联得到增强。这项研究表明，FPI的功能性能可以通过共价连接超声组合来扩展，从而扩大其在食品部门和其他领域的潜在用途。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.