Fluorescence Turn-On Sensing of Leucine Using Bimetallic Cu-Ag Nanoclusters: A Potential Non-Invasive Biomarker for Cancer Detection.

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2025-04-21 Epub Date: 2025-03-25 DOI:10.1021/acsabm.5c00102

Geneva Indongo, Susan Varghese, Merin K Abraham, Greeshma Rajeevan, Arathy B Kala, Dheyaa Mohammed Dhahir, Dr Sony George

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Abstract

This study investigates the use of bimetallic copper-silver nanoclusters (Cu-AgNCs) for fluorescence turn-on sensing of leucine, a potential biomarker for cancer detection. These nanoclusters exhibit high fluorescence tunability and specificity, with Fe³⁺ serving as a quencher to facilitate leucine detection. The fluorescence recovery mechanism is attributed to the interaction of leucine with Fe³⁺, alleviating the quenching effect on the metal nanoclusters. This bimetallic nanocluster is a promising platform for biomarker identification in cancer diagnosis. The fluorescence enhancement upon leucine binding provides a measurable signal, confirming the feasibility of these nanoclusters as noninvasive sensors for cancer biomarkers. The sensor achieves a detection limit of 0.58 μM and demonstrates a linear response within the range of 110-657 μM. This approach offers a promising method for noninvasive cancer diagnostics using saliva and urine samples. Additionally, the method's reproducibility and robustness further support its potential in clinical applications, providing a cost-effective and accessible technique for early cancer detection.

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利用双金属Cu-Ag纳米团簇对亮氨酸的荧光开启感测：一种潜在的非侵入性癌症检测生物标志物。

本研究研究了双金属铜银纳米团簇（cu - agnc）在亮氨酸荧光开启传感中的应用，亮氨酸是一种潜在的癌症检测生物标志物。这些纳米团簇具有高荧光可调性和特异性，Fe3+作为猝灭剂促进亮氨酸检测。荧光恢复机制归因于亮氨酸与Fe3+的相互作用，减轻了对金属纳米团簇的猝灭作用。这种双金属纳米簇是一种很有前途的癌症诊断生物标志物鉴定平台。亮氨酸结合后的荧光增强提供了可测量的信号，证实了这些纳米团簇作为癌症生物标志物的无创传感器的可行性。该传感器的检测限为0.58 μM，线性响应范围为110 ~ 657 μM。这种方法为使用唾液和尿液样本进行非侵入性癌症诊断提供了一种很有前途的方法。此外，该方法的可重复性和稳健性进一步支持了其在临床应用中的潜力，为早期癌症检测提供了一种具有成本效益和可获得的技术。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.