A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh
{"title":"Effect of Human Macrophage Conditioned Media on TGF-β-Induced Differentiation of Lung Fibroblasts.","authors":"A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh","doi":"10.1007/s10517-025-06353-7","DOIUrl":null,"url":null,"abstract":"<p><p>We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"442-446"},"PeriodicalIF":0.6000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06353-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/26 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
