Selenomethionine attenuates Klebsiella pneumoniae-induced jejunal injury in rabbits by inhibiting the TLR4/NF-κB pathway

IF 3.5 3区医学 Q3 IMMUNOLOGY Microbial pathogenesis Pub Date : 2025-03-25 DOI:10.1016/j.micpath.2025.107510

Lihui Shi, Yumei Liu, Jianing Wang, Chenhao Chang, Xuemin Zhu, Lan Wei, Xiaoguang Chen, Ziqiang Zhang

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Abstract

Klebsiella pneumoniae (KP) infection often causes diarrhoea and intestinal barrier damage in young rabbits. The objective of this study was to explore whether selenomethionine (SeMet) can attenuate the jejunal injury caused by KP in rabbits. Therefore, we investigated the protective effect of SeMet by performing haematoxylin-eosin (HE), alcian blue periodic acid Schiff (AB-PAS), proliferating nuclear antigen (PCNA), TUNEL and immunofluorescence staining. In addition, the concentrations of Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Tumor necrosisfactor-α (TNF-α) and Interleukin-10 (IL-10) in the jejunal tissue were detected by enzyme-linked immunosorbent assay (ELISA). The results showed that after KP infection, the productivity of rabbits decreased, and the mucosal barrier of the jejunum was damaged. Moreover, KP induced jejunal inflammation, activated the TLR4/NF-κB signalling pathway, and promoted the expression of the IL-1β, IL-6, and TNF-α. In addition, KP increased the apoptotic response of intestinal cells and upregulated the expression of caspase-3 and caspase-9. SeMet pretreatment significantly decreased the degree of intestinal epithelial cell apoptosis. Therefore, we showed that SeMet can reduce inflammation and enhance intestinal barrier function to improve the production performance of rabbits infected with KP.

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硒代蛋氨酸通过抑制TLR4/NF-κB通路减轻肺炎克雷伯菌诱导的家兔空肠损伤

肺炎克雷伯菌（KP）感染通常会导致幼兔腹泻和肠屏障损伤。本研究旨在探讨硒代蛋氨酸（SeMet）是否能减轻KP引起的家兔空肠损伤。为此，我们采用血红素-伊红（HE）、阿利新蓝周期性酸希夫（AB-PAS）、增殖核抗原（PCNA）、TUNEL和免疫荧光染色等方法研究了SeMet的保护作用。采用酶联免疫吸附法（ELISA）检测大鼠空肠组织中白细胞介素-1β (IL-1β)、白细胞介素-6 （IL-6）、肿瘤坏死因子-α (TNF-α)和白细胞介素-10 （IL-10）的浓度。结果表明，感染KP后，家兔生产能力下降，空肠黏膜屏障受损。此外，KP诱导空肠炎症，激活TLR4/NF-κB信号通路，促进IL-1β、IL-6和TNF-α的表达。此外，KP增加了肠细胞的凋亡反应，上调了caspase-3和caspase-9的表达。SeMet预处理可显著降低肠上皮细胞凋亡程度。因此，我们发现SeMet可以减轻炎症，增强肠道屏障功能，从而提高KP感染家兔的生产性能。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)