Mechanistic exploration of licorice reconciling Medicine:Huangqi Guizhi Wuwu decoction and Shaoyao Gancao decoction compatibility as an example

IF 5.4 2区医学 Q1 CHEMISTRY, MEDICINAL Journal of ethnopharmacology Pub Date : 2025-04-25 Epub Date: 2025-03-26 DOI:10.1016/j.jep.2025.119708

Shuwan Tang , Shengnan Fu , Yunfeng Zheng , Xiaolan Cheng , Peng Cao , Cunyu Li , Guoping Peng

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Abstract

Ethnopharmacological relevance

Glycyrrhiza uralensis Fisch. (GU) is a pivotal botanical in traditional Chinese medicine (TCM), because of its ability to reconcile various herbs, and its crucial role in numerous formulas. Huanggui Shaogan decoction (HSGD) is an empirical formula, developed by Huangqi Guizhi Wuwu decoction (HGD) and Shaoyao Gancao decoction (SGD), and prepared by adding GU to HGD. However, the mechanisms of GU reconciling medicine remain incompletely characterized.

Aim of the study

This study aimed to explain mechanisms of GU reconciling medicine based on the differences of components, pharmacological efficacy, and the existence of the components.

Methods

Differential components between HSGD and HGD were systematically identified using LC‒MS/MS coupled with chemometric analysis. The existence states and binding affinities of these differential components were further characterized via ultrafiltration separation. The therapeutic potential of HSGD was validated in a murine model of oxaliplatin-induced peripheral neuropathy (OIPN).

Results

Twenty-two differential chemical components between HSGD and HGD were identified, including flavonoids, saponins, gingerol, and monoglycoside. The transmittance of flavonoids and gingerols increased in HSGD. However, the transmittance of astragalus saponins decreased, which may be due to micelle association and the increase in molecular clusters. HSGD could enhance the mechanical pain threshold, alleviate cold nociceptive hypersensitivity, relieve dorsal root ganglia neuron injury, and decrease the expression of nerve growth factor, 5-hydroxytryptamine, substance P, and calcitonin gene-related peptide better. The differential correlation analysis revealed the relationship between differential components and pharmacological indicators. The above results indicated that different herbs combinations had a greater impact on the dissolution and molecular state of the components of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao.

Conclusion

The study highlights the solubilizing effect of GU within HSGD, and it also improves the efficacy of the treatment of OIPN, which underpins its compatibility rationality. It provided an inspiration for the study of other TCM formulas.

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甘草调和医学的机理探讨：以黄芪桂枝五物汤与少药甘草汤配伍为例

乌拉尔甘草的民族药理学意义。由于其调和各种草药的能力以及在许多方剂中的重要作用，它是传统中医（TCM）中的关键植物。黄芪减肝汤（HSGD）是由黄芪桂枝五物汤（HGD）和少药肝草汤（SGD）共同研制而成的经验配方。然而，谷氨酸调和药物的机制尚不完全清楚。研究目的本研究旨在从成分差异、药理功效、成分存在等方面来解释谷草调和药物的作用机制。方法采用LC-MS /MS结合化学计量学分析，系统鉴定两种产品的差异成分。通过超滤分离进一步表征了这些差异组分的存在状态和结合亲和力。HSGD的治疗潜力在奥沙利铂诱导的周围神经病变（OIPN）小鼠模型中得到了验证。结果鉴定出黄酮类化合物、皂苷类化合物、姜辣素类化合物和单糖苷类化合物共22种。黄酮和姜辣素的透光率在HSGD中升高。而黄芪总皂苷的透光率下降，可能与胶束缔合和分子簇增加有关。HSGD能提高机械痛阈，减轻冷伤害性超敏反应，减轻背根神经节神经元损伤，较好地降低神经生长因子、5-羟色胺、P物质、降钙素基因相关肽的表达。差异相关分析揭示了差异成分与药理指标之间的关系。上述结果表明，不同中药组合对黄芪各成分的溶出度和分子状态影响较大。知母。蒙古变种（大）萧。结论本研究突出了GU在HSGD中的增溶作用，同时也提高了治疗OIPN的疗效，支持了其配伍合理性。为其他中药方剂的研究提供了启示。

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.