Synthesize of NiSe2/Mo-MOF nanocomposite for sensitive determination of antiretroviral agent dolutegravir in pharmaceutical formulations and biological fluids

Abstract

The precise detection of antiretroviral agents, such as Dolutegravir (DTG), is essential for effective HIV treatment and optimal patient management. This study presents the synthesis of NiSe₂/Mo-MOF nanocomposite and its application as a modifier for the accurate detection of DTG in pharmaceutical formulations and biological fluids. NiSe₂ nanocrystals were synthesized using a modified hydrothermal method and subsequently incorporated into a Mo-MOF matrix through a solvothermal process. Characterization of the nanocomposite was performed using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and electrochemical impedance spectroscopy (EIS). Electrochemical investigations demonstrated that the NiSe₂/Mo-MOF sensor exhibited excellent sensitivity, selectivity, and reproducibility, with a low detection limit of 3.68 nM for DTG. The superior performance of the sensor can be attributed to the synergistic interaction between NiSe₂ and Mo-MOF. This study demonstrates the potential of NiSe₂/Mo-MOF nanocomposites as efficient, reliable, and cost-effective electrochemical sensors, providing a promising platform for drug monitoring in clinical and pharmaceutical applications.