Characterization of dengue patients in Vietnam: Clinical, virological, and IL-10 profiles during 2021- 2022 outbreaks.

IF 3.4 2区医学 Q1 PARASITOLOGY PLoS Neglected Tropical Diseases Pub Date : 2025-03-28 eCollection Date: 2025-03-01 DOI:10.1371/journal.pntd.0012954

Do Duc Anh, Lara Vugrek, Nguyen Trong The, Nourhane Hafza, Truong Nhat My, Le Thi Kieu Linh, Do Huy Loc, Jonas Schmidt-Chanasit, Nguyen Linh Toan, Peter G Kremsner, Le Huu Song, Thirumalaisamy P Velavan

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Abstract

Background: The pathogenesis of dengue is attributed to a complex interaction between the dengue virus (DENV) and the host immune system. The aim of this study is to investigate the clinical, virological, and Interleukin-10 (IL-10) profiles of dengue patients in Vietnam from two consecutive outbreaks in 2021 and 2022.

Methods: A total of n=306 dengue patients were examined, who were clinically stratified according to dengue without warning signs (DF; n=178), dengue with warning signs (DWS; n=115) and severe dengue (SD; n=13). Patients were screened for dengue, Zika and chikungunya viruses. DENV were subjected to serotype specific real-time RT-PCR. Interleukin-10 (IL-10) levels were measured by ELISA, and IL-10 promoter variants (-1082G/A; -819C/T; -592C/A) were genotyped by direct Sanger sequencing to determine a possible association with susceptibility to dengue and disease severity.

Results: No chikungunya or Zika viruses were detected. Patients were infected by one of the three different DENV serotypes (DENV-1, -2, -4). Plasma IL-10 levels were significantly elevated in patients (DF vs. DWS, p=0.004; DF vs. SD, p=0.001; DWS vs. SD, p=0.015). While the IL-10 allele -819C contributed to an increased risk of dengue (OR = 1.5, 95% CI = 1.1-2.0, p=0.04), genotype -1082GA showed a protective role against the disease (OR = 0.45, 95% CI = 0.27-0.72, p=0.009), and allele -1082G showed a protective role against DWS (OR = 0.44, 95% CI = 0.22-0.81, p=0.049). Also, the IL-10 GTA (-1082G/-819T/-592A) haplotype was observed to confer protection (OR = 0.31, 95% CI = 0.14-0.67, p< 0.003).

Conclusion: While DENV-1 and DENV-2 were the predominant serotypes in circulation, plasma IL-10 levels and IL-10 promoter variants were also significantly associated with dengue and its severity.

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越南登革热病人的特征：2021-2022年登革热疫情爆发期间的临床、病毒学和IL-10概况。

背景：登革热的发病机制归因于登革热病毒（DENV）与宿主免疫系统之间复杂的相互作用。本研究的目的是调查2021年和2022年越南两次连续暴发的登革热患者的临床、病毒学和白细胞介素-10 （IL-10）谱。方法：共检查n=306例登革热患者，按登革热无预警体征(DF；n=178)，有警告迹象的登革热(DWS；n=115)和重症登革热(SD；n = 13)。对患者进行了登革热、寨卡病毒和基孔肯雅病毒筛查。对DENV进行血清型特异性实时RT-PCR检测。ELISA法检测白细胞介素-10 （IL-10）水平，检测IL-10启动子变异(-1082G/A；-819 c / T;-592C/A)通过直接Sanger测序进行基因分型，以确定与登革热易感性和疾病严重程度的可能关联。结果：未检出基孔肯雅病毒和寨卡病毒。患者感染三种不同DENV血清型（DENV-1、-2、-4）中的一种。患者血浆IL-10水平显著升高(DF vs DWS, p=0.004；DF vs. SD, p=0.001；DWS vs. SD, p=0.015)。IL-10等位基因-819C与登革热风险增加有关（OR = 1.5, 95% CI = 1.1-2.0, p=0.04），基因型-1082GA对登革热有保护作用（OR = 0.45, 95% CI = 0.27-0.72, p=0.009），等位基因-1082G对DWS有保护作用（OR = 0.44, 95% CI = 0.22-0.81, p=0.049）。此外，IL-10 GTA （-1082G/-819T/-592A）单倍型被观察到具有保护作用（OR = 0.31, 95% CI = 0.14-0.67, p< 0.003）。结论：虽然DENV-1和DENV-2是血液循环中的主要血清型，但血浆IL-10水平和IL-10启动子变异也与登革热及其严重程度显著相关。

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PLoS Neglected Tropical Diseases PARASITOLOGY-TROPICAL MEDICINE

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10.50%

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723

期刊介绍： PLOS Neglected Tropical Diseases publishes research devoted to the pathology, epidemiology, prevention, treatment and control of the neglected tropical diseases (NTDs), as well as relevant public policy. The NTDs are defined as a group of poverty-promoting chronic infectious diseases, which primarily occur in rural areas and poor urban areas of low-income and middle-income countries. Their impact on child health and development, pregnancy, and worker productivity, as well as their stigmatizing features limit economic stability. All aspects of these diseases are considered, including: Pathogenesis Clinical features Pharmacology and treatment Diagnosis Epidemiology Vector biology Vaccinology and prevention Demographic, ecological and social determinants Public health and policy aspects (including cost-effectiveness analyses).