MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair.

IF 4.8 3区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in Bioengineering and Biotechnology Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI:10.3389/fbioe.2025.1554292

Shanyu Lu, Zhenyu Liu, Meiling Qi, Haocheng Zhen, Jing Luo, Yingchao Wang, Le Chang, Xiaolong Bai, Yingguang Jiao, Xinyao Chen, Junping Zhen

{"title":"MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair.","authors":"Shanyu Lu, Zhenyu Liu, Meiling Qi, Haocheng Zhen, Jing Luo, Yingchao Wang, Le Chang, Xiaolong Bai, Yingguang Jiao, Xinyao Chen, Junping Zhen","doi":"10.3389/fbioe.2025.1554292","DOIUrl":null,"url":null,"abstract":"Objective: This study aimed to evaluate the effectiveness of bone marrow mesenchymal stem cells (BMSCs) combined with sodium alginate scaffolds in repairing knee cartilage defects in New Zealand rabbits. Additionally, it assessed the potential of functional magnetic resonance imaging (fMRI) for non-invasive monitoring of the dynamic repair process.Methods: Rabbits were randomly divided into four groups: Group A (control), Group B (sodium alginate scaffold), Group C (BMSCs-sodium alginate scaffold), and Group D (USPIO-labeled BMSCs-sodium alginate scaffold). A cartilage defect model was created, and the respective materials were implanted into the defect regions. T2 mapping MRI was performed at weeks 1, 2, and 4 post-surgery to evaluate the repair process, followed by histological analysis to confirm the outcomes.Results: BMSCs significantly promoted cartilage defect repair and accelerated the degradation of sodium alginate scaffolds. Macroscopic and histological evaluations revealed repair tissue formation in Groups C and D by week 1, with most defect regions filled with new cartilage by week 4. T2 mapping analysis showed a gradual decline in T2 values in Group B, a more pronounced decrease in Group C, and consistently lower T2 values in Group D compared to Group C, with a slow upward trend over time.Conclusion: This study demonstrated that BMSCs exhibit significant regenerative potential for cartilage defect repair. USPIO labeling enables non-invasive, dynamic monitoring of the repair process without adverse effects on cell viability or differentiation. These findings provide experimental evidence supporting the application of BMSCs combined with magnetic labeling technology in cartilage regeneration.","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1554292"},"PeriodicalIF":4.8000,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955663/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Bioengineering and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3389/fbioe.2025.1554292","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This study aimed to evaluate the effectiveness of bone marrow mesenchymal stem cells (BMSCs) combined with sodium alginate scaffolds in repairing knee cartilage defects in New Zealand rabbits. Additionally, it assessed the potential of functional magnetic resonance imaging (fMRI) for non-invasive monitoring of the dynamic repair process.

Methods: Rabbits were randomly divided into four groups: Group A (control), Group B (sodium alginate scaffold), Group C (BMSCs-sodium alginate scaffold), and Group D (USPIO-labeled BMSCs-sodium alginate scaffold). A cartilage defect model was created, and the respective materials were implanted into the defect regions. T2 mapping MRI was performed at weeks 1, 2, and 4 post-surgery to evaluate the repair process, followed by histological analysis to confirm the outcomes.

Results: BMSCs significantly promoted cartilage defect repair and accelerated the degradation of sodium alginate scaffolds. Macroscopic and histological evaluations revealed repair tissue formation in Groups C and D by week 1, with most defect regions filled with new cartilage by week 4. T2 mapping analysis showed a gradual decline in T2 values in Group B, a more pronounced decrease in Group C, and consistently lower T2 values in Group D compared to Group C, with a slow upward trend over time.

Conclusion: This study demonstrated that BMSCs exhibit significant regenerative potential for cartilage defect repair. USPIO labeling enables non-invasive, dynamic monitoring of the repair process without adverse effects on cell viability or differentiation. These findings provide experimental evidence supporting the application of BMSCs combined with magnetic labeling technology in cartilage regeneration.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

uspio标记骨髓间充质干细胞联合海藻酸盐支架修复软骨缺损的MRI监测。

目的：探讨骨髓间充质干细胞（BMSCs）联合海藻酸钠支架修复新西兰兔膝关节软骨缺损的效果。此外，它还评估了功能磁共振成像（fMRI）在动态修复过程的非侵入性监测中的潜力。方法：将家兔随机分为A组（对照组）、B组（海藻酸钠支架）、C组（骨髓间充质干细胞-海藻酸钠支架）、D组（uspio标记的骨髓间充质干细胞-海藻酸钠支架）。建立软骨缺损模型，将相应材料植入缺损区域。术后1周、2周和4周进行T2定位MRI评估修复过程，随后进行组织学分析以确认结果。结果：骨髓间充质干细胞显著促进软骨缺损修复，加速海藻酸钠支架的降解。肉眼和组织学检查显示，C组和D组在第1周形成修复组织，大部分缺损区域在第4周被新软骨填充。T2制图分析显示，B组T2值逐渐下降，C组下降更为明显，D组T2值持续低于C组，且随时间推移呈缓慢上升趋势。结论：本研究表明骨髓间充质干细胞在软骨缺损修复中具有显著的再生潜力。USPIO标记可以实现对修复过程的非侵入性动态监测，而不会对细胞活力或分化产生不利影响。这些发现为骨髓间充质干细胞联合磁标记技术在软骨再生中的应用提供了实验证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Frontiers in Bioengineering and Biotechnology Chemical Engineering-Bioengineering

CiteScore

8.30

自引率

5.30%

发文量

2270

审稿时长

12 weeks

期刊介绍： The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs. In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.