Mass photometry as a fast, facile characterization tool for direct measurement of mRNA length.

Abstract

Oligonucleotide integrity is a critical quality attribute for many new therapeutic modalities. Current assays often measure attributes such as length using capillary electrophoresis or liquid chromatography. The length is then corroborated with sequencing data to ensure oligonucleotide quality. An orthogonal measure to these classical separations is to measure intact mass, which traditional mass spectrometry cannot. Herein, we report the use of mass photometry to directly measure RNA length using RNA ladders as a calibrant.