{"title":"Preclinical Evaluation of HSP90 Noninvasive Imaging in Colorectal Cancer Diagnosis","authors":"Lenan Zou, Qi Chang, Chengwei Chen, Ao Li, Yang Luo, Xiaohui Wang, Xue Li, Zihan Wu, Mengyao Zhou, Haoran Xu, Hui Wang, Zhihao Han, Yueqing Gu","doi":"10.1021/acs.analchem.4c05864","DOIUrl":null,"url":null,"abstract":"Heat shock protein 90 (HSP90) is a powerful molecular chaperone responsible for the stability and integrity of various client proteins, including various transcription factors, kinases, and steroid hormone proteins, among others. Overexpression of HSP90 is closely related to cancer, immune diseases, and neurodegenerative diseases. However, there are few effective fluorescent probes for HSP90. Therefore, we synthesized a near-infrared fluorescent probe YQHSI-MPA-1 targeting HSP90. The probe was later structurally modified to improve the targeting specificity and accuracy in vivo as YQHSI-MPA-2. Furthermore, the application values of YQHSI-MPA-2 in situ CRC, AOM-DSS-induced CRC, and various metastasis models were evaluated. The results showed that YQHSI-MPA-2 had excellent specificity and high tumor contrast. In conclusion, YQHSI-MPA-2 is an ideal tool for tumor detection and surgical navigation.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c05864","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Heat shock protein 90 (HSP90) is a powerful molecular chaperone responsible for the stability and integrity of various client proteins, including various transcription factors, kinases, and steroid hormone proteins, among others. Overexpression of HSP90 is closely related to cancer, immune diseases, and neurodegenerative diseases. However, there are few effective fluorescent probes for HSP90. Therefore, we synthesized a near-infrared fluorescent probe YQHSI-MPA-1 targeting HSP90. The probe was later structurally modified to improve the targeting specificity and accuracy in vivo as YQHSI-MPA-2. Furthermore, the application values of YQHSI-MPA-2 in situ CRC, AOM-DSS-induced CRC, and various metastasis models were evaluated. The results showed that YQHSI-MPA-2 had excellent specificity and high tumor contrast. In conclusion, YQHSI-MPA-2 is an ideal tool for tumor detection and surgical navigation.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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