Ahmed F Hikal, Shaohua Zhao, Steven Foley, Ashraf A Khan
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引用次数: 0
Abstract
Plazomicin, a next-generation aminoglycoside, was recently approved by the U.S. Food and Drug Administration for the treatment of multidrug-resistant complicated urinary tract infections. It is highly effective against most aminoglycoside-modifying enzymes (AMEs). Here, we report that Salmonella enterica strains containing the newly identified gentamicin resistance gene (grdA) are highly resistant to plazomicin. Heterologous expression of grdA in Escherichia coli ΔtolC resulted in plazomicin resistance with minimum inhibitory concentration (MIC) > 256 µg/mL. These findings reveal that GrdA confers significantly higher resistance to plazomicin than the previously known plazomicin-resistant AMEs AA (2)-Ia and APH (2″)-Iva.
Plazomicin是新一代氨基糖苷类药物，最近被美国食品和药物管理局批准用于治疗多重耐药的复杂尿路感染。它对大多数氨基糖苷修饰酶（AMEs）非常有效。在这里，我们报道了含有新发现的庆大霉素耐药基因（grdA）的肠沙门氏菌菌株对plazomicin具有高度耐药性。grdA在大肠杆菌ΔtolC中异源表达，对plazomicin产生耐药性，最低抑菌浓度（MIC）为bb0 ~ 256µg/mL。这些发现表明，GrdA对plazomicin的耐药性明显高于先前已知的plazomicin耐药AMEs AA (2)-Ia和APH（2″）-Iva。
期刊介绍：
Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.
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