{"title":"Rapid and High-Yielding Purification of DNA Self-Assembled Structures by Aqueous Two-Phase System.","authors":"Marcos K Masukawa, Masahiro Takinoue","doi":"10.1007/978-1-0716-4394-5_2","DOIUrl":null,"url":null,"abstract":"<p><p>Aqueous two-phase systems (ATPS) of dextran and polyethylene glycol (PEG) enable the purification of DNA structures such as DNA origami and DNA nanotubes in times as short as 10 min. This method, which has recovery yields >90% for a typical DNA origami, owes its efficiency to the highly selective partition of the DNA structures in the dextran phase of these emulsions. This purification method is carried out in conditions that promote the structural stability of these structures, making it particularly suitable for DNA nanotechnology. In this protocol, we will describe the materials and methods for purifying DNA origami and quantifying the purification yield by agarose electrophoresis and image analysis.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2901 ","pages":"13-25"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-0716-4394-5_2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
Aqueous two-phase systems (ATPS) of dextran and polyethylene glycol (PEG) enable the purification of DNA structures such as DNA origami and DNA nanotubes in times as short as 10 min. This method, which has recovery yields >90% for a typical DNA origami, owes its efficiency to the highly selective partition of the DNA structures in the dextran phase of these emulsions. This purification method is carried out in conditions that promote the structural stability of these structures, making it particularly suitable for DNA nanotechnology. In this protocol, we will describe the materials and methods for purifying DNA origami and quantifying the purification yield by agarose electrophoresis and image analysis.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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