RNAi assay in the 28-spotted ladybeetle Henosepilachna vigintioctopunctata suggests nuclear receptor HR3 as a potential molecular target for pest control

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2025-04-03 DOI:10.1002/ps.8810

Zhaoyang Li, Yalin Ruan, Chuqiao Zhou, Junna Liu, Yajie Wang, June-Sun Yoon, Xuguo Zhou, Youjun Zhang, Chunxiao Yang, Huipeng Pan

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Abstract

BACKGROUND

Nuclear hormone receptors play crucial roles in embryonic development, neural regulation, metabolism, and organogenesis in insects. This study aimed to investigate the expression and function of the nuclear hormone receptor 3 (HR3) in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata.

RESULTS

Our findings revealed that HvHR3 was expressed at all developmental stages, with the highest expression levels in the 1st instar larvae, 3rd instar larvae, and pupa. HvHR3 silencing in both the 1st and 3rd instar larvae resulted in high mortality rates. Reduced HvHR3 expression in the 3rd instar larvae led to developmental arrest and failure to molt, accompanied by a down-regulation of 20E-related genes. Furthermore, HvHR3 silencing in the 3rd instar larvae resulted in a decrease in the 20E titer. Silencing HvHR3 in adult females significantly impaired ovarian development, resulting in reduced ovary size and absence of mature oocytes. Additionally, the eggs produced by these females had partially ruptured shells and failed to maintain normal spindle shape.

CONCLUSION

These findings indicate that HvHR3 is a critical regulator of development, molting, and reproduction, underscoring its potential as a molecular target for RNA interference-based biopesticides aimed at controlling this pest. © 2025 Society of Chemical Industry.

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对28斑瓢虫的RNAi分析表明，核受体HR3可能是害虫防治的潜在分子靶点。

背景：核激素受体在昆虫的胚胎发育、神经调节、代谢和器官发生中起着重要作用。本研究旨在探讨核激素受体3 （HR3）在28斑瓢虫（Henosepilachna vigintioctopunctata）中的表达和功能。结果：HvHR3在各发育阶段均有表达，在1龄幼虫、3龄幼虫和蛹中表达量最高。1龄和3龄幼虫的HvHR3沉默导致高死亡率。HvHR3在3龄幼虫中的表达降低导致发育停滞和不能蜕皮，并伴有20e相关基因的下调。此外，HvHR3在3龄幼虫中的沉默导致20E滴度下降。在成年女性中，沉默HvHR3会显著损害卵巢发育，导致卵巢大小缩小和成熟卵母细胞缺失。此外，这些雌性产卵的蛋壳部分破裂，无法保持正常的纺锤形。结论：这些研究结果表明，HvHR3是一种重要的发育、蜕皮和繁殖调节因子，强调了其作为基于RNA干扰的生物农药的分子靶点的潜力，旨在控制害虫。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.