Virulence plasmids in edema disease: Insights from whole-genome analysis of porcine O139:H1 Shiga toxin-producing Escherichia coli (STEC) strains.

IF 4.8 2区医学 Q2 IMMUNOLOGY Frontiers in Cellular and Infection Microbiology Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1528408

Ali Nemati, Federica Gigliucci, Stefano Morabito, Mahdi Askari Badouei

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Abstract

This study investigates the plasmid sequences of porcine O139:H1 Shiga toxin-producing Escherichia coli (STEC) responsible for Edema Disease (ED). Whole-genome analysis reveals significant similarities between these strains and known plasmids, notably pW1316-2, which harbors key virulence genes like hemolysin (hlyA, hlyB) and adhesion factors (aidA-I, faeE). These genes contribute to the cytotoxicity and host colonization associated with ED. Additionally, similarities to plasmids from Shigella flexneri 2a highlight potential associations in virulence gene regulation, particularly via the Hha-H-NS complex. The identification of sequences resembling plasmid pB71 raises serious concerns about the emergence of highly pathogenic strains, as it includes tetracycline resistance genes (tetA, tetC, tetR). This research emphasizes the role of plasmid-like sequences in ED pathogenesis, indicating important implications for swine industry management and public health.

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水肿病的毒力质粒：来自猪O139:H1产志贺毒素大肠杆菌（STEC）菌株全基因组分析的见解

本研究研究了引起猪水肿病（ED）的产志贺毒素大肠杆菌（STEC） O139:H1的质粒序列。全基因组分析揭示了这些菌株与已知质粒之间的显著相似性，特别是pW1316-2，其中含有溶血素（hlyA, hlyB）和粘附因子（aida - 1, faeE）等关键毒力基因。这些基因有助于与ED相关的细胞毒性和宿主定植。此外，与福氏志贺氏菌2a质粒的相似性突出了毒力基因调控的潜在关联，特别是通过ha- h - ns复合物。与质粒pB71相似的序列的鉴定引起了人们对出现高致病性菌株的严重关注，因为它包含四环素耐药基因（tetA, tetC, tetR）。本研究强调了质粒样序列在ED发病机制中的作用，对养猪业管理和公共卫生具有重要意义。

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来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.