{"title":"Flavin-based probe for real-time monitoring of hypochlorous acid dynamics in live cells†","authors":"Harsha Gopal Agrawal, Pravin Shankar Giri, Tanima Sahoo, Subha Narayan Rath and Ashutosh Kumar Mishra","doi":"10.1039/D4TB02727B","DOIUrl":null,"url":null,"abstract":"<p >The present study introduces TPA-vinylene-flavin (<strong>TVF</strong>) as a flavin-based turn-on fluorescent probe. <strong>TVF</strong> effectively detects HOCl, as evaluated by the solution phase studies with a detection limit of 0.36 μM. This probe shows excellent biocompatibility and rapid cellular internalization, making it suitable for real-time monitoring of HOCl fluctuations in both physiological and pathological conditions. Furthermore, the <strong>TVF</strong> probe exhibits specific mitochondrial localization and selectively detects HOCl in both endogenous and exogenous contexts within live cells. It demonstrates excellent sensitivity to HOCl concentrations over time, enabling precise tracking of dynamic fluctuations, which is critical for understanding its role in cellular processes and oxidative stress-related pathologies.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 17","pages":" 5109-5116"},"PeriodicalIF":6.1000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02727b?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb02727b","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
The present study introduces TPA-vinylene-flavin (TVF) as a flavin-based turn-on fluorescent probe. TVF effectively detects HOCl, as evaluated by the solution phase studies with a detection limit of 0.36 μM. This probe shows excellent biocompatibility and rapid cellular internalization, making it suitable for real-time monitoring of HOCl fluctuations in both physiological and pathological conditions. Furthermore, the TVF probe exhibits specific mitochondrial localization and selectively detects HOCl in both endogenous and exogenous contexts within live cells. It demonstrates excellent sensitivity to HOCl concentrations over time, enabling precise tracking of dynamic fluctuations, which is critical for understanding its role in cellular processes and oxidative stress-related pathologies.
期刊介绍：
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive：
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices
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