Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp.

IF 4.9 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Microbial Cell Factories Pub Date : 2025-04-04 DOI:10.1186/s12934-025-02703-2

Xiaowen Cui, Tingting Chen, Yizhen Meng, Xueshan Pan, Ruizhe Wu, Yinghua Lu, Chuanyi Yao, Xihuang Lin, Xueping Ling

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Abstract

The polyunsaturated fatty acid (PUFA) metabolism of Schizochytrium, an excellent oil-producing microorganism, is closely related to phosphatidylcholine (PC) synthesis, which favors the migration and accumulation of docosahexaenoic acid (DHA). Phosphocholine cytidylyltransferase (CCT), a key enzyme involved in PC synthesis, profoundly impacts lipid metabolism in plants; however, few studies have focused on CCT in microorganisms. We investigated the effects of CCT overexpression on lipid metabolism in Schizochytrium sp. CCT overexpression slightly inhibited cell growth, but significantly promoted total lipid synthesis. Compared to the wild-type strain, PUFA content and DHA production in the CCT-overexpressing strain (SR21-CCT) increased by about 49% and 46%, respectively. Analysis of phospholipids and quantitative real-time PCR revealed that CCT overexpression enhanced phospholipid synthesis, especially by strengthening glycerophosphorylcholine acylation and de novo PC synthesis pathways, which promote DHA esterification to PC and DHA accumulation in triacylglycerols. This study helps decipher the mechanism correlating phospholipid metabolism and DHA production.

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schizochtrium sp.磷脂酰转移酶（CCT）过表达合成磷脂酰胆碱，确定磷脂与二十二碳六烯酸连接的代谢机制。

Schizochytrium是一种优良的产油微生物，其多不饱和脂肪酸（PUFA）代谢与磷脂酰胆碱（PC）合成密切相关，有利于二十二碳六烯酸（DHA）的迁移和积累。磷脂酰转移酶（Phosphocholine cytidyltransferase， CCT）是磷脂合成的关键酶，对植物脂质代谢有重要影响；然而，很少有研究关注微生物中的CCT。我们研究了CCT过表达对Schizochytrium sp.脂质代谢的影响。CCT过表达轻微抑制细胞生长，但显著促进总脂质合成。与野生型菌株相比，过表达cct的菌株（SR21-CCT）的PUFA含量和DHA产量分别增加了49%和46%。磷脂分析和实时荧光定量PCR显示，CCT过表达增强了磷脂合成，特别是通过加强甘油酰胆碱酰化和PC合成途径，促进DHA酯化成PC和DHA在三酰基甘油中的积累。这项研究有助于破译磷脂代谢和DHA生产的相关机制。

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来源期刊

Microbial Cell Factories 工程技术-生物工程与应用微生物

CiteScore

9.30

自引率

4.70%

发文量

235

审稿时长

2.3 months

期刊介绍： Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems