Y. Yang, G. Sun, X. Ge, C. Xia, C. Zhang, Y. Zhu, Z. Wang
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引用次数: 0
Abstract
Enhancing cobalt tolerance is crucial for microbial adsorption and recovery of cobalt from wastewater. In this study, metabolic engineering of Saccharomyces cerevisiae was conducted to systematically investigate the effects of modifying cell wall and membrane components and regulating intracellular antioxidant substances glutathione (GSH) and S-adenosylmethionine (SAM) on the improvement of cobalt tolerance. The cobalt tolerance was increased to 157% by overexpression of the erg4 and erg6 genes in the ergosterol metabolic pathways. In the strain where the key cell wall synthesis gene fks2 was knocked out, the cobalt tolerance was increased by 63%. However, this study also found that there was no direct correlation between the intracellular GSH and SAM levels and the cobalt tolerance of the strain. This study provides theoretical support for further improving the tolerance of S. cerevisiae to cobalt ion in the future.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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