Green synthesized zinc oxide nanoparticle with Mentha pulegium L. extracts in A549 cell line, characterization, biological activities, Genotoxicity in comet test and SMART assay in Drosophila melanogaster.

Abstract

In this study, zinc oxide nanoparticles (ZnO NPs) were obtained by green synthesis method using extracts prepared from Mentha pulegium L. species in order to investigate the cytotoxic, genotoxic and antimicrobial activities of nanoparticles. For the characterization of these nanoparticles, UV-Vis spectrophotometer, FT-IR, XRD and SEM analysis methods were used. For cell culture studies were carried out to determine the cytotoxic and genotoxic activities of ZnO NPs obtained by green synthesis with A549 cell line, which is a lung cancer cell. In the genotoxicity results determined by the Comet method, the highest DNA damage was seen at a concentration of 3.75 mg/mL. The genotoxic activity of different concentrations (0.1, 1, 5, 10 mM) of ZNO NPs were evaluated with SMART assay on Drosophila melanogaster. According to results ZNO NPs applications were found to be similar to the control group in all doses. On the other hand, in order to determine the antimicrobial activity, Escherichia coli (ATTC 25922), Staphylococcus aureus (ATTC 29213), Candida albicans (ATTC 90028), Klebsiella pneumoniae (ATTC 700603) and Salmonella enteritidis (ATTC 13076) microorganisms were cultured and disc diffusion test method was applied. In the disc diffusion test, dose application was made in the range of 15.6-500 mg/mL concentrations and it was observed that inhibition zone was formed at all concentrations.