{"title":"Changes in 5-hydroxytryptamine and its metabolite, 5-hydroxyindole-acetic acid, levels in the brain of rats administered with methotrexate.","authors":"Sachiko Hiraide, Takuji Machida, Mina Yamada, Honoka Furukawa, Takahiro Yamamoto, Naoya Hamaue, Kenji Iizuka","doi":"10.2220/biomedres.46.77","DOIUrl":null,"url":null,"abstract":"<p><p>During the delayed emesis phase, methotrexate tended to increase 5-hydroxytryptamine (5-HT) levels in midbrain and significantly increased 5-hydroxyindole-acetic acid (5-HIAA), a metabolite of 5-HT, levels in the area postrema and in the cerebral cortex in rats. However, methotrexate did not increase mRNA expression of 5-HT biosynthesis/metabolism enzyme in the area postrema. The increased 5-HIAA levels in the area postrema may result from enhanced intestinal 5-HT synthesis, with 5-HIAA reaching the area postrema via circulation. Additionally, the rise in cortical 5-HIAA levels might reflect stress-related increases in brain tryptophan influx.</p>","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 2","pages":"77-81"},"PeriodicalIF":1.3000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Research-tokyo","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2220/biomedres.46.77","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
During the delayed emesis phase, methotrexate tended to increase 5-hydroxytryptamine (5-HT) levels in midbrain and significantly increased 5-hydroxyindole-acetic acid (5-HIAA), a metabolite of 5-HT, levels in the area postrema and in the cerebral cortex in rats. However, methotrexate did not increase mRNA expression of 5-HT biosynthesis/metabolism enzyme in the area postrema. The increased 5-HIAA levels in the area postrema may result from enhanced intestinal 5-HT synthesis, with 5-HIAA reaching the area postrema via circulation. Additionally, the rise in cortical 5-HIAA levels might reflect stress-related increases in brain tryptophan influx.
期刊介绍：
Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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