Cumulus cells conditioned medium facilitates germ cell differentiation from human embryonic stem cells: An experimental study.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY International Journal of Reproductive Biomedicine Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI:10.18502/ijrm.v23i1.18186

Somayyeh Sadat Tahajjodi, Ehsan Farashahi Yazd, Azam Agharahimi, Fatemeh Akyash, Fatemeh Hajizadeh-Tafti, Jalal Golzadeh, Reza Aflatoonian, Behrouz Aflatoonian

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Abstract

Background: Studies have shown thatembryonic stem cells can differentiate into germ cells either spontaneously or directed by the defined factors or by applying a co-culture method or conditioned medium of stromal cells.

Objective: Here, Yazd4 (human embryonic stem cells [hESC] line; 46, XX) was used to evaluate the effect of human cumulus cells conditioned medium (CCCM) on in vitro germ cell production and development.

Materials and methods: In this experimental study 2 different complete media were applied, Dulbecco's modified eagle's medium + 20% fetal bovin serum and embryoid body (EB) medium (KnockOut serum replacement- hESC without basal fibroblast growth factor). Thus, there are 2 control groups for spontaneous differentiation (SD) (SD-EB and SD-DM) and 2 types of CCCM (CCCM-DM and CCCM-EB) as test groups. 40% dilution of the conditioned medium was applied according to previous reports.

Results: Reverse transcription-quantitative polymerase chain reaction and immunofluorescent staining assays revealed that between groups with similar complete medium, CCCM provides a better condition for in vitro germ cell differentiation from hESCs. Moreover, a comparison between the 2 types of complete media showed that EB medium is more supportive than Dulbecco's modified eagle's medium +20% fetal bovin serum.

Conclusion: CCCM with EB medium as a complete medium provides a better condition for in vitro germ cell differentiation from hESCs.

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积云细胞条件培养基促进人胚胎干细胞生殖细胞分化的实验研究。

背景：研究表明，胚胎干细胞可以自发地向生殖细胞分化，也可以在特定因素的引导下，或通过基质细胞的共培养方法或条件培养基向生殖细胞分化。目的：建立人胚胎干细胞Yazd4 （hESC）系；46， XX)评价了人积云细胞条件培养基（CCCM）对体外生殖细胞产生和发育的影响。材料和方法：本实验采用2种不同的完全培养基，Dulbecco改良鹰培养基+ 20%胎牛血清和胚状体（EB）培养基（KnockOut血清替代-不含基底成纤维细胞生长因子的hESC）。因此，有2个自发分化（SD）对照组（SD- eb和SD- dm）和2种CCCM类型（CCCM- dm和CCCM- eb）作为实验组。根据先前的报道，将条件培养基稀释40%。结果：逆转录-定量聚合酶链反应和免疫荧光染色结果显示，在完全培养基相似的两组间，CCCM为hESCs体外生殖细胞分化提供了较好的条件。两种完全培养基的比较表明，EB培养基比Dulbecco改良鹰培养基+20%胎牛血清的支持作用更强。结论：以EB培养基为完整培养基的CCCM为hESCs体外生殖细胞分化提供了较好的条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Reproductive Biomedicine OBSTETRICS & GYNECOLOGY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

16 weeks

期刊介绍： The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.