Masterful ecofriendly maintainable spectrophotometric techniques for considering indacaterol acetate and mometasone furoate: full evaluation and comparison of their greenness, whiteness, and blueness profiles†

Abstract

People of all ages may suffer from asthma. According to recent research, those who have poorly controlled asthma may be more susceptible to hospitalization and severe symptoms from any respiratory infection, particularly severe ones like the COVID-19 and human metapneumovirus (HMPV) viruses, which could result in the death of these critically ill people. Therefore, a study of the mixture of indacaterol acetate and mometasone furoate which is used in the new remarkable anti-asthmatic Breezhaler will produce substantial gains for the scientific field. A binary blend of (INDA) indacaterol acetate (1.00–30.00) μg mL⁻¹, and (MOM) mometasone furoate (1.00–40.00) μg mL⁻¹ was estimated over these ranges using six sensitive, neoteric, rapid, frugal, universal, and explicit spectrophotometric methods using the most satisfactory green solvent: ethanol. These methods are the dual wavelength method, ratio subtraction method, factorized absorbance difference method, advanced absorbance subtraction method, derivative ratio method, and derivative amplitude factor method. These procedures have been sensitively and effectively utilized for the analysis of INDA and MOM in commercial Breezhaler capsules with multiple ratios for the first time with low LOD (0.186 and 0.172) and LOQ values (0.565 and 0.520) for INDA and MOM, respectively, superior to those in other published spectrophotometric work, and they were validated in accordance with ICH specifications. Additionally, they delivered 98–102% recovery percentages and showed RSD% of less than 2 for the studied drugs, confirming their specificity. Ecological aspects of the developed work were evaluated and gave satisfactory results using several evaluation tools [such as NEMI, Eco-Scale (=93), GAPI, AGREE (=76)] for the greenness aspect, RGB (=96.3) for the whiteness aspect, and BAGI (=90) for the blueness aspect. Additionally, an environmental assessment and statistical comparisons were made using Student's t-test and the F-test with the published HPLC method and those methods currently in use.