HPLC-DAD and HPLC-ELSD Profiling of Various Parts of Selected Ficus Species

IF 2.5 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry & Biodiversity Pub Date : 2025-04-09 DOI:10.1002/cbdv.202403260
Milan Malaník, Terézia Jedináková, Tereza Stiskálková, Denisa Witteková, Anežka Chlebková, Karel Šmejkal
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Abstract

Due to the scarcity of chemical data related to some Ficus species, forty-three ethanolic extracts were obtained from various parts of twelve Ficus species encompassing understudied ones. Thus, these were analyzed by high-performance liquid chromatography-diode array detection (HPLC-DAD) and HPLC-evaporative light scattering detection (HPLC-ELSD) to explore their phytochemical profiles. Overall, most of the extracts contain predominantly compounds lacking chromophore visible only in HPLC-ELSD chromatograms. However, HPLC-DAD analyses revealed furanocoumarins psoralen and bergapten to be dominant in extracts of all parts of F. carica and F. pumila, together with the extract of the leaves of F. cyathistipula. Some extracts (e.g., those from F. microcarpa and F. lyrata) displayed only peaks corresponding to chlorogenic acid and its derivatives. Remarkably, the extract of the roots of F. cyathistipula is rich in prenylated isoflavones. This study brings an overview of dominant compounds present in selected Ficus species and points out huge differences in their phytochemical profiles.

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HPLC-DAD和HPLC-ELSD对榕树不同部位的分析。
由于某些榕树物种的相关化学数据稀缺,我们从 12 个榕树物种的不同部位获得了 43 种乙醇提取物,其中包括研究不足的物种。因此,对这些提取物进行了 HPLC-DAD 和 HPLC-ELSD 分析,以探索其植物化学特征。总体而言,大多数提取物主要含有仅在 HPLC-ELSD 色谱图中可见的缺乏发色团的化合物。然而,HPLC-DAD 分析表明,呋喃香豆素类化合物补骨脂素和佛手苷在 F. carica 和 F. pumila 所有部位的提取物以及 F. cyathistipula 的叶提取物中都占主导地位。有些提取物(如小叶榕和小叶榕的提取物)只显示出与绿原酸及其衍生物相对应的峰值。值得注意的是,F. cyathistipula 的根提取物富含前炔基异黄酮。本研究概述了部分榕属植物中存在的主要化合物,并指出了其植物化学特征的巨大差异。
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来源期刊
Chemistry & Biodiversity
Chemistry & Biodiversity 环境科学-化学综合
CiteScore
3.40
自引率
10.30%
发文量
475
审稿时长
2.6 months
期刊介绍: Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.
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