Dihydromyricetin ameliorates hyperuricemia through inhibiting uric acid reabsorption

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-04-09 DOI:10.1002/jsfa.14126

Ze-Rui Sun, Hui-Zhen Peng, Mao-Si Fan, Dong Chang, Ming-Yue Wang, Meng-Fei An, Li-Juan Zhang, Rui Zan, Jun Sheng, Yun-Li Zhao, Xuan-Jun Wang

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Abstract

BACKGROUND

Hyperuricemia (HUA) is a chronic disease caused by abnormal purine metabolism with high prevalence. Dihydromyricetin (DMY) is a natural flavonoid that is abundant in plants, such as vine tea, grapes and bayberry. DMY has been shown to possess multiple biological properties, but its anti-HUA effect remains underexplored. In the present study, the regulatory effects of DMY on HUA and its complications and mechanism were investigated.

RESULTS

DMY (10 and 20 μmol L⁻¹) treatment significantly reduced xanthine oxidase (XOD) expression and uric acid (UA) synthesis in normal human liver cell strain cells, and intraperitoneal administration of DMY (100 mg kg⁻¹) also significantly reduced serum UA and the expression of hepatic XOD in HUA mice. After DMY treatment for 12 consecutive days, the uricosuric protein, ATP-binding cassette subfamily G member 2, was upregulated, and reabsorption proteins, including urate transporter 1 and glucose transporter 9, were downregulated, which was consistent with the results of monosodium urate-induced HUA in human renal tubular epithelial cell line and human colon adenocarcinoma cell line cell models. In addition, DMY significantly ameliorated HUA-induced renal injury, and foot edema induced by monosodium urate. The nucleotide-binding oligomerization domain-like receptor family containing pyrin domain 3 (NLRP3) inflammasome was activated in HUA mice as evidenced by upregulation of NLRP3, caspase-1, ACS, TNF-α and IL-1β in the kidney and foot, which was significantly suppressed by DMY treatment.

CONCLUSION

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二氢杨梅素通过抑制尿酸重吸收改善高尿酸血症。

背景：高尿酸血症（HUA）是一种由嘌呤代谢异常引起的慢性疾病，发病率较高。二氢杨梅素（DMY）是一种富含植物的天然类黄酮，如藤茶、葡萄和杨梅。DMY已被证明具有多种生物学特性，但其抗hua作用仍未得到充分研究。本研究探讨了DMY对HUA的调控作用及其并发症和机制。结果：DMY（10和20 μmol L-1）处理显著降低正常人肝细胞株细胞黄嘌呤氧化酶（XOD）表达和尿酸（UA）合成，腹腔注射DMY （100 mg kg-1）也显著降低HUA小鼠血清UA和肝脏XOD表达。DMY连续治疗12天后，尿素蛋白atp结合盒亚家族G成员2上调，尿酸转运蛋白1和葡萄糖转运蛋白9下调，这与尿酸钠诱导的人肾小管上皮细胞系和人结肠腺癌细胞系细胞模型的HUA结果一致。此外，DMY可显著改善hua所致的肾损伤和尿酸钠所致的足部水肿。含pyrin结构域3的核苷酸结合寡聚结构域样受体家族（NLRP3）炎症小体在HUA小鼠中被激活，其证据是肾和足的NLRP3、caspase-1、ACS、TNF-α和IL-1β上调，而DMY治疗显著抑制了这种上调。结论：综上所述，这些发现提示DMY可能在实验性HUA中起重要作用。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.