LINC00426 promotes the progression of atherosclerosis by regulating miR-873-5p/SRRM2 axis

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2025-04-14 DOI:10.1016/j.cyto.2025.156938

Bo Zhou , Lu Gan , Pimo Zhou , Tai Yang , Fang Tang , Peng Jin , Ping Jin , Jiulin Chen

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Abstract

Background

Atherosclerosis (AS) is a disease that occurs in the great arteries and is the main cause of cardiovascular disease and death.

Objective

To investigate the clinical significance of LINC00426 in AS and to investigate that LINC00426 regulates PDGF-BB-induced proliferation, migration, invasion and inflammatory response of vascular smooth muscle cells (VSMCs) by modulating miR-873-5p/SRRM2 axis.

Methods

The expression of LINC00426 was detected using RT-qPCR. The diagnostic role of LINC00426 in AS was analyzed with ROC curves. CCK-8 assay was used to measure cell proliferation, and transwell assay was used to measure cell migration and invasion ability. The targeted binding relationship between LINC00426 and miR-873-5p, miR-873-5p and SRRM2 was detected using dual-luciferase reporter gene assay. The concentration of proinflammatory factors was detected by using ELISA kit.

Result

The expression of LINC00426 was increased in patients with AS, and LINC00426 had a diagnostic role in AS. In addition, LINC00426 regulated PDGF-BB-induced proliferation, migration, invasion, and inflammation of VSMCs by regulating miR-873-5p/SRRM2 axis.

Conclusion

LINC00426 may function as a biomarker for the diagnosis and treatment of AS.

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LINC00426通过调节miR-873-5p/SRRM2轴促进动脉粥样硬化的进展

动脉粥样硬化（AS）是一种发生在大动脉的疾病，是心血管疾病和死亡的主要原因。目的探讨LINC00426在AS中的临床意义，探讨LINC00426是否通过调节miR-873-5p/SRRM2轴调控pdgf - bb诱导的血管平滑肌细胞（VSMCs）的增殖、迁移、侵袭及炎症反应。方法采用RT-qPCR检测LINC00426的表达。采用ROC曲线分析LINC00426对AS的诊断作用。CCK-8法检测细胞增殖，transwell法检测细胞迁移和侵袭能力。采用双荧光素酶报告基因法检测LINC00426与miR-873-5p、miR-873-5p与SRRM2的靶向结合关系。采用ELISA试剂盒检测促炎因子浓度。结果LINC00426在AS患者中表达升高，对AS有诊断作用。此外，LINC00426通过调节miR-873-5p/SRRM2轴调控pdgf - bb诱导的VSMCs的增殖、迁移、侵袭和炎症。结论linc00426可作为as诊断和治疗的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.