{"title":"Cover Feature: Boronic Acid-Linked Apo-Zinc Finger Protein for Ubiquitin Delivery in Live Cells (ChemBioChem 7/2025)","authors":"Dr. Pritam Ghosh, Prof. Dr. Oliver Seitz","doi":"10.1002/cbic.202580702","DOIUrl":null,"url":null,"abstract":"<p>Benzoboroxole facilitates the delivery of ubiquitin to living cells by utilizing the apo-zinc finger protein domain and thereby converts the apo-zinc finger protein (apo-ZFP) into a cell-penetrating peptide (CPP), despite being inherently a non-CPP. The introduction of point mutations within the skeletal structure, along with the incorporation of benzoboroxole into the lysine side chain, further enhances the efficacy of apo-ZFP. Cellular delivery was monitored using fluorescence microscopy and validated through flow cytometry. More details can be found inarticle 10.1002/cbic.202401040 by Pritam Ghosh and Oliver Seitz. Parts of the image were created with BioRender.com.\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure>\n </p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 7","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202580702","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202580702","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
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Abstract
Benzoboroxole facilitates the delivery of ubiquitin to living cells by utilizing the apo-zinc finger protein domain and thereby converts the apo-zinc finger protein (apo-ZFP) into a cell-penetrating peptide (CPP), despite being inherently a non-CPP. The introduction of point mutations within the skeletal structure, along with the incorporation of benzoboroxole into the lysine side chain, further enhances the efficacy of apo-ZFP. Cellular delivery was monitored using fluorescence microscopy and validated through flow cytometry. More details can be found inarticle 10.1002/cbic.202401040 by Pritam Ghosh and Oliver Seitz. Parts of the image were created with BioRender.com.
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ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).
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