J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY Nano Letters Pub Date : 2025-04-18 DOI:10.1021/acs.nanolett.5c01012

Gan Zhang, Keyang Lai, Lilin Zhang, Xiaocui Lai, Shengliang Deng, Juan Peng, Weihua Lai

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Abstract

The application of conventional lateral flow immunoassay (LFIA) remains challenging due to its single signal readout and poor sensitivity. In this work, we developed J-aggregated fluorescence nanoparticles (JNPs) with multichromatic performance and employed them as reporters for the simultaneous and sensitive detection of T-2 and AFB1 toxins on the LFIA platform. Upon the strong confinement effect of nanoparticles, the emission region of JNPs was red-shifted from ∼545 to ∼620 nm, exhibiting a strong J-aggregation phenomenon. Subsequently, two JNPs with green emission and red emission were employed for the development of multiplexed LFIA platform (JNPs–LFIA) for on-site quantitative detection of T-2 and AFB1 toxins, with the limits of detection of 0.645 ng mL^–1 and 0.0035 ng mL^–1, which were 3.5-fold and 12.2-fold lower than those of AuNPs-LFIA, respectively. Overall, this work provides an efficient strategy for the design of multichromatic immunoprobes and promotes the development of reporters in biosensing application.

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具有多色性能的j聚集荧光纳米粒子使LFIA平台敏感

传统的侧流免疫分析法（LFIA）由于信号读数单一且灵敏度较差，其应用仍然具有挑战性。在这项工作中，我们开发了具有多色性能的j聚集荧光纳米颗粒（JNPs），并将其作为报告者，在LFIA平台上同时灵敏地检测T-2和AFB1毒素。在纳米粒子的强约束作用下，JNPs的发射区从~ 545 nm红移到~ 620 nm，表现出强烈的j聚集现象。随后，采用两种具有绿色和红色发射特性的JNPs构建了多重LFIA平台（JNPs -LFIA），用于T-2和AFB1毒素的现场定量检测，其检出限分别为0.645 ng mL-1和0.0035 ng mL-1，分别比AuNPs-LFIA低3.5倍和12.2倍。总之，本研究为多色免疫探针的设计提供了一种有效的策略，并促进了报告蛋白在生物传感应用中的发展。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.