Reverse-Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Viral Detection — A Case Study
Ashley Hassman, Renée Richie Casey, Colby Rouchka, Diego Sunino, Felix Veloz Espinal, Mona Youssef
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引用次数: 0
Abstract
An assay and protocol framework are provided for utilizing reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) at the point-of-care for diagnosing and monitoring a hypothetical zoonotic viral outbreak in a resource-limited area. This manuscript utilizes a previously published decision tree algorithm to determine an appropriate molecular diagnostic point-of-care test that can effectively address the outbreak presented in the hypothetical case study. © 2025 Wiley Periodicals LLC.
用于病毒检测的反转录酶环路介导等温扩增（RT-LAMP）--案例研究
本文提供了一种检测方法和方案框架，用于在护理点利用反转录酶环介导等温扩增（RT-LAMP）技术诊断和监测资源有限地区的假想人畜共患病毒疫情。本手稿利用以前发表的决策树算法来确定适当的分子诊断护理点测试，以有效解决假设案例研究中提出的疫情问题。© 2025 Wiley Periodicals LLC.
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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