Efficient fluorescence and electrochemiluminescence dual-signal au nanoclusters-based portable antibiotic testing platform with super-wide detection range

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2025-04-23 DOI:10.1016/j.foodchem.2025.144339

Ying Zhou , Senfeng Liu , Xinlei Yan , Yunying Wei , Liang Ma , Ruo Yuan

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Abstract

The convenient detection of tetracycline (TC) residues has attracted considerable attention because irrational use of TC causes food pollution damaging the human health. Herein, a point-of-care testing (POCT) platform is established for the sensitive and specific determination of TC in a polydopamine-functionalised Eppendorf tube. In particular, TC can be efficiently recognised by an aptamer–antibody chimera to trigger visual fluorescence (FL) and electrochemiluminescence (ECL) ‘dual-signal’ response of gold nanoclusters. Consequently, the biosensor exhibits a wide detection range spanning from 5 fM to 1 μM, with low detection limits of 73 fM (visual FL) and 2.3 fM (ECL). Hence, it can be said that the POCT platform is superior to the enzyme-linked immunosorbent assay. The strategy utilises the strength of FL, i.e. visualisation, and that of ECL, i.e. high sensitivity, providing a guiding approach for the development of POCT in food security and environmental monitoring.

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基于超宽检测范围的高效荧光和电化学发光双信号 Au 纳米团簇便携式抗生素检测平台

由于不合理使用四环素（TC）会造成食品污染，损害人类健康，因此方便地检测四环素（TC）残留引起了广泛关注。本文建立了一种床旁检测（POCT）平台，可在多巴胺功能化的艾本多夫试管中灵敏、特异地检测四环素。特别是，TC 可被一种适配体-抗体嵌合体有效识别，从而触发金纳米团簇的视觉荧光（FL）和电化学发光（ECL）"双信号 "反应。因此，该生物传感器的检测范围从 5 fM 到 1 μM，检测限低至 73 fM（视觉荧光）和 2.3 fM（电化学发光）。因此，可以说 POCT 平台优于酶联免疫吸附测定法。该策略利用了 FL 的优势（即可视化）和 ECL 的优势（即高灵敏度），为在食品安全和环境监测领域开发 POCT 提供了一种指导方法。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.